Localization of tyrosine hydroxylase mRNA in the axons of the hypothalamo-neurohypophysial system

Brain Research. Molecular Brain Research
T SkutellaG F Jirikowski

Abstract

With in situ hybridization we examined the localization of mRNA coding for tyrosine hydroxylase (TH) in the rat hypothalamo-neurohypophysial system (HNS) under conditions of acute osmotic stress. Fifteen min after salt loading, hybridization signal of TH mRNA could be located in the magnocellular hypothalamic nuclei and in the median eminence (ME). In untreated animals, TH mRNA was detected only in the ME. In osmotically challenged animals that had been pretreated with colchicine, signals for TH mRNA remained confined to the ME, while pretreatment of salt loaded rats with a polymerase II transcription inhibitor resulted in labelling of the magnocellular perikarya but a decrease of the hybridization signal in the ME. Our results suggest that also TH mRNA is among the RNAs which are axonally transported in the HNS. TH mRNA can probably be stored in axons of the hypothalamo-neurohypophysial tract, to be transported retrogradely and translated upon certain stimuli.

References

Dec 1, 1979·The Journal of Endocrinology·G ClarkeL P Merrick
Oct 1, 1991·Hormone and Metabolic Research = Hormon- Und Stoffwechselforschung = Hormones Et Métabolisme·G F JirikowskiJ D Caldwell
Oct 1, 1990·Proceedings of the National Academy of Sciences of the United States of America·G F JirikowskiF E Bloom
Jan 1, 1985·Proceedings of the National Academy of Sciences of the United States of America·B GrimaJ Mallet
Oct 1, 1986·Physiology & Behavior·J P Kroon, A L Riley
Aug 20, 1984·The Journal of Comparative Neurology·V Chan-PalayS L Palay
Apr 1, 1983·The Journal of Histochemistry and Cytochemistry : Official Journal of the Histochemistry Society·G Pelletier
May 1, 1982·Proceedings of the National Academy of Sciences of the United States of America·R ZandomeniR Weinmann
Jan 1, 1993·Journal of Neural Transmission. General Section·T SkutellaG F Jirkowski

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Citations

Aug 1, 1996·Histochemistry and Cell Biology·R W Dirks
Aug 2, 1996·Neuroscience Letters·O D WeinerG D Bittner
Feb 20, 2010·Molecular & Cellular Proteomics : MCP·Dianna E Willis, Jeffery L Twiss
Jun 28, 2001·Physiological Reviews·J P BurbachH Gainer
Aug 1, 1994·Trends in Neurosciences·W J BrewsterD R Tomlinson

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