Abstract
We have investigated the functional interchangeability of EF hands I and III or II and IV, which occupy structurally analogous positions in the native I-II and III-IV EF hand pairs of calmodulin. Our approach was to functionally characterize four engineered proteins, made by replacing in turn each EF hand in one pair by a duplicate of its structural analog in the other. In this way functional determinants we define as unique were localized to the component EF hands in each pair. Replacement of EF hand I by III reduces calmodulin-dependent activation of cerebellar nitric oxide synthase activity by 50%. Replacement of EF hand IV by II reduces by 60% activation of skeletal muscle myosin light chain kinase activity. There appear to be no major unique determinants for activation of these enzyme activities in the other EF hands. Replacement of EF hand III by I or IV by II reduces by 50-80% activation of smooth muscle myosin light chain kinase activity, and replacement of EF hand I by III or II by IV reduces by 90% activation of this enzyme activity. Thus, calmodulin-dependent activation of each of the enzyme activities examined, even the closely related kinases, is dependent upon a distinct pattern of unique determinants in the four ...Continue Reading
References
Dec 1, 1992·Proceedings of the National Academy of Sciences of the United States of America·K McMillanB S Masters
Oct 2, 1992·Science·D R KnightonB E Kemp
Aug 28, 1992·Science·W E MeadorF A Quiocho
May 1, 1992·Science·M IkuraA Bax
Dec 1, 1991·Journal of Molecular Graphics·C C HuangR Langridge
Jul 23, 1991·Biochemistry·J Mackall, C B Klee
Jan 8, 1991·Biochemistry·S LinseS Forsén
Feb 1, 1990·Trends in Biochemical Sciences·K T O'Neil, W F DeGrado
Sep 1, 1990·The Journal of Cell Biology·M O ShoemakerD M Watterson
Nov 1, 1989·Trends in Neurosciences·A PersechiniR H Kretsinger
Dec 30, 1986·Biochemistry·V GuerrieroA R Means
Nov 5, 1988·Journal of Molecular Biology·Y S BabuW J Cook
Aug 5, 1980·Biochemistry·T H Crouch, C B Klee
Dec 8, 1995·The Journal of Biological Chemistry·Z SuS E George
Jan 1, 1995·Annual Review of Biophysics and Biomolecular Structure·A Crivici, M Ikura
Jun 12, 1995·FEBS Letters·K SiivariH J Vogel
Jan 1, 1995·Trends in Biochemical Sciences·P JamesE Carafoli
Jun 7, 1994·Biochemistry·P M Stemmer, C B Klee
Jan 1, 1993·Protein Engineering·G J Barton
Jan 9, 1996·Biochemistry·A PersechiniR J Paresi
Nov 1, 1995·Protein Science : a Publication of the Protein Society·W A FindlayP M Bayley
Mar 22, 1996·Cell·J GoldbergJ Kuriyan
Apr 30, 1996·Biochemistry·K NakashimaM Yazawa
Aug 9, 1996·The Journal of Biological Chemistry·A PersechiniR J Paresi
Citations
Mar 17, 2004·Biochimica Et Biophysica Acta·Cheng YangKrzysztof Kuczera
Aug 1, 1997·Current Opinion in Structural Biology·Y Lu, J S Valentine
Feb 14, 2002·Trends in Cardiovascular Medicine·Anthony Persechini, Paul M Stemmer
May 20, 1999·The Biochemical Journal·J A CobbD M Roberts
Aug 6, 2000·The Biochemical Journal·S H LeeM J Cho
Jul 17, 2001·Immunology·R SchmalzigaugM W Berchtold
Mar 3, 2009·The Journal of Biological Chemistry·Quang-Kim TranAnthony Persechini
Oct 2, 2002·Journal of Biomolecular Structure & Dynamics·Cheng Yang, Krzysztof Kuczera
Dec 7, 2007·PLoS Computational Biology·Michael Graupner, Nicolas Brunel
Feb 24, 2016·Archives of Biochemistry and Biophysics·Jonathan P DavisShane D Walton
Aug 5, 2008·Biochimica Et Biophysica Acta·Qin WangRongqing Zhang
Mar 29, 2006·Proteins·Assaf GanothMenachem Gutman
Oct 23, 1997·Cell Calcium·A PersechiniV A Romoser
Dec 14, 1999·The Journal of Biological Chemistry·R KondoJ D Johnson
Aug 22, 2001·The Journal of Biological Chemistry·A J CarideJ T Penniston
Aug 22, 2002·The Journal of Biological Chemistry·Ingo PaarmannManfred Konrad
Mar 6, 1999·The Journal of Biological Chemistry·A Persechini, B Cronk
Mar 7, 2017·Frontiers in Plant Science·Shane D WaltonJonathan P Davis
Feb 8, 2000·The Journal of Biological Chemistry·A PersechiniP M Stemmer
Jun 20, 2006·The Journal of Biological Chemistry·Linda J Roman, Bettie Sue S Masters
Sep 20, 2017·Journal of Inorganic Biochemistry·Hiroshi Kawasaki, Robert H Kretsinger