Localization of urokinase to focal adhesions by human fibrosarcoma cells synthesizing recombinant vitronectin

Biochemistry and Cell Biology = Biochimie Et Biologie Cellulaire
S A WilcoxPaula J McKeown-Longo

Abstract

Cell surface plasminogen activators have been proposed to participate in cell migration and invasion by activating both intracellular signaling pathways and extracellular proteolysis. Urokinase-type plasminogen activator (uPA) is secreted from many cell types and localizes to focal contact areas when cells are seeded onto the plasma protein vitronectin. Induction of vitronectin synthesis during migration of neural crest cells and growth of certain tumors suggests that the de novo synthesis and deposition of vitronectin into the tissue matrix may remodel the matrix to provide an environment suitable for cell migration and (or) tumor invasion. To investigate the effects of vitronectin secretion and matrix deposition on the localization and activity of cell-associated uPA, HT-1080 fibrosarcoma cells were transfected with the Rc/CMV expression vector containing a vitronectin cDNA insert and stable cell lines expressing vitronectin were selected. Vitronectin-secreting cells were allowed to attach and spread on collagen- and fibronectin-coated substrates. Within 6 h, vitronectin was detected on the substrate; vitronectin synthesis was accompanied by the clustering of both the alpha v beta 5 vitronectin receptor and uPA into vinculin-...Continue Reading

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Citations

Jun 16, 2001·Journal of Cellular Biochemistry·P A UnderwoodL Cubeddu
Oct 29, 2000·Cell Adhesion and Communication·S A Wilcox-AdelmanP J McKeown-Longo
Mar 9, 2007·Matrix Biology : Journal of the International Society for Matrix Biology·Roman M SalasznykPaula J McKeown-Longo
Mar 21, 2008·European Journal of Cell Biology·Chris D Madsen, Nicolai Sidenius

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