Nov 1, 1991

Localized adherence by enteropathogenic Escherichia coli is an inducible phenotype associated with the expression of new outer membrane proteins

The Journal of Experimental Medicine
J Vuopio-Varkila, G K Schoolnik

Abstract

Enteropathogenic Escherichia coli grow as discrete colonies on the mucous membranes of the small intestine. A similar pattern can be demonstrated in vitro; termed localized adherence (LA), it is characterized by the presence of circumscribed clusters of bacteria attached to the surfaces of cultured epithelial cells. The LA phenotype was studied using B171, an O111:NM enteropathogenic E. coli (EPEC) strain, and HEp-2 cell monolayers. LA could be detected 30-60 min after exposure of HEp-2 cells to B171. However, bacteria transferred from infected HEp-2 cells to fresh monolayers exhibited LA within 15 min, indicating that LA is an inducible phenotype. Induction of the LA phenotype was found to be associated with de novo protein synthesis and changes in the outer membrane proteins, including the production of a new 18.5-kD polypeptide. A partial NH2-terminal amino acid sequence of this polypeptide was obtained and showed it to be identical through residue 12 to the recently described bundle-forming pilus subunit of EPEC. Expression of the 18.5-kD polypeptide required the 57-megadalton enteropathogenic E. coli adherence plasmid previously shown to be required for the LA phenotype in vitro and full virulence in vivo. This observation...Continue Reading

Mentioned in this Paper

Alkalescens-Dispar Group
Pathogenicity
Squamous Transitional Epithelial Cell Count
Small Intestinal Wall Tissue
Outer Membrane Lipoproteins, Bacterial
Protein Biosynthesis
Entire Small Intestine
Tubocin
Neoplasm of Uncertain or Unknown Behavior of Small Intestine
Virulence

About this Paper

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