Localized effect of treated wastewater effluent on the resistome of an urban watershed.

GigaScience
Christopher N ThorntonWilliam J Brazelton

Abstract

Wastewater treatment is an essential tool for maintaining water quality in urban environments. While the treatment of wastewater can remove most bacterial cells, some will inevitably survive treatment to be released into natural environments. Previous studies have investigated antibiotic resistance within wastewater treatment plants, but few studies have explored how a river's complete set of antibiotic resistance genes (the "resistome") is affected by the release of treated effluent into surface waters. Here we used high-throughput, deep metagenomic sequencing to investigate the effect of treated wastewater effluent on the resistome of an urban river and the downstream distribution of effluent-associated antibiotic resistance genes and mobile genetic elements. Treated effluent release was found to be associated with increased abundance and diversity of antibiotic resistance genes and mobile genetic elements. The impact of wastewater discharge on the river's resistome diminished with increasing distance from effluent discharge points. The resistome at river locations that were not immediately downstream from any wastewater discharge points was dominated by a single integron carrying genes associated with resistance to sulfonami...Continue Reading

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Datasets Mentioned

BETA
PRJNA562643

Methods Mentioned

BETA
ICEs
electrophoresis
ICE

Software Mentioned

ggplot2
Picard Tools
Nonpareil
BLAST +
stats
BBmap
Megahit
CASAVA
MAFFT
Mix

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