Localized expression of angiopoietin 1 and 2 may explain unique characteristics of the rat testicular microvasculature

Biology of Reproduction
Stina Häggström RudolfssonA Bergh

Abstract

The testicular vasculature is unique in several ways. The unfenestrated endothelial cells constitute one part of the blood-testis barrier, and testicular microvessels are normally resistant to inflammation mediators. At the same time that angiogenic factors and inflammation mediators are constitutively produced, the proportion of proliferating endothelial cells is considerably higher than in other organs, but new blood vessels are not formed. Hormonal stimulation of the testis with hCG increase endothelial cell proliferation, vascular permeability, and sensitivity to locally injected inflammation mediators. In the present study, we examined whether local expression of angiopoietin (ang) 1, an inhibitor of vascular leakage and sprouting angiogenesis, and its antagonist, ang 2, could be involved in establishing this vascular phenotype. Using reverse transcription-polymerase chain reaction and immunohistochemistry, we demonstrate that testicular vascular endothelial growth factor-A (VEGF-A), ang 1, ang 2, and the ang-receptor tie 2 are expressed in the testis and that hormonal stimulation with hCG is accompanied by increased expression of VEGF-A and ang 2. The ang 1 protein is expressed in testicular microvessels under basal condi...Continue Reading

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Citations

Sep 12, 2006·Journal of Huazhong University of Science and Technology. Medical Sciences = Hua Zhong Ke Ji Da Xue Xue Bao. Yi Xue Ying De Wen Ban = Huazhong Keji Daxue Xuebao. Yixue Yingdewen Ban·Yuanhang LuZhonghua Zhu
Oct 17, 2003·Biology of Reproduction·Stina Häggström RudolfssonAnders Bergh
Dec 15, 2010·Infection and Immunity·Miranda S OakleySanjai Kumar
Aug 28, 2009·Journal of Experimental & Clinical Cancer Research : CR·Oscar ArrietaJosé Luis Aguilar
Mar 14, 2007·Molecular and Cellular Endocrinology·K ReisingerM Zygmunt
May 15, 2009·Clinical and Experimental Allergy : Journal of the British Society for Allergy and Clinical Immunology·H KanazawaK Hirata
Sep 10, 2011·Pediatric Pulmonology·Diego G PeroniGiorgio L Piacentini
Apr 16, 2015·International Journal of Endocrinology·Shan ChenYumei Wang
Nov 21, 2007·Molecular Reproduction and Development·Eugene D AlbrechtGerald J Pepe
Apr 8, 2017·International Journal of Endocrinology·Ana Silvia CorlanMarius Raica

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Methods Mentioned

BETA
PCR
electrophoreses
dissection

Software Mentioned

LightCycler
SPSS

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