Localized maternal proteins in Xenopus revealed by subtractive immunization

Developmental Biology
James M DenegreKimberly L Mowry

Abstract

It has long been appreciated that the localization of cytoplasmic determinants in the egg can provide the foundation for patterning in the embryo. Differences in cell fate among the early blastomeres are thus a consequence of asymmetric distributions of informational molecules prior to fertilization. The frog egg has a single axis of asymmetry present prior to fertilization, the animal/vegetal axis, and the localization of developmental information appears to be polarized along this axis. Such developmental information can be localized as either RNA or protein; localized RNAs are well documented in the Xenopus oocyte, and some are thought to play roles in axial patterning. While it is apparent that not all of the localized maternal components are RNAs, much less is known about maternal proteins that might be localized in the egg. In the present study, we have taken a novel approach to identify localized maternal proteins within the Xenopus egg. Using a subtractive immunization strategy, we have generated monoclonal antibodies which recognize antigens that are restricted to the vegetal cortex of fertilized eggs. Analysis of biogenesis during oogenesis reveals two distinct patterns of localization to the cortex. At least three of...Continue Reading

References

Sep 1, 1979·Proceedings of the National Academy of Sciences of the United States of America·H TowbinJ Gordon
Jul 1, 1977·Developmental Biology·T L Moen, M Namenwirth
Jun 26, 1987·Journal of Immunological Methods·W D Matthew, A W Sandrock
Oct 1, 1994·Developmental Biology·M W Klymkowsky, A Karnovsky
Apr 21, 1995·Cell·D St Johnston
Oct 28, 1994·Science·D S Kessler, D A Melton
Dec 1, 1993·Developmental Biology·R P ElinsonC Forristall
Feb 1, 1997·BioEssays : News and Reviews in Molecular, Cellular and Developmental Biology·M Kühl, D Wedlich
Dec 1, 1969·Wilhelm Roux' Archiv Für Entwicklungsmechanik Der Organismen·P D Nieuwkoop
Sep 1, 1991·Roux's Archives of Developmental Biology : the Official Organ of the EDBO·Akio S SuzukiAsako Hirakawa

❮ Previous
Next ❯

Citations

Jun 15, 1997·European Journal of Biochemistry·M FrieserL M Sorokin
Dec 9, 1998·Proceedings of the National Academy of Sciences of the United States of America·J M DenegreK L Mowry
Apr 21, 2004·The Journal of Cell Biology·Tracy L KressKimberly L Mowry
Dec 17, 2014·Zygote : the Biology of Gametes and Early Embryos·Arkadiy A Reunov, Yulia A Reunova
Mar 19, 2005·Genes to Cells : Devoted to Molecular & Cellular Mechanisms·Keiichi SakakibaraYasuo Fukami
Apr 22, 1999·Microscopy Research and Technique·P ChangE Houliston
Oct 16, 2002·World Journal of Gastroenterology : WJG·Lian-Jun Yang, Wen-Liang Wang
Jun 26, 2007·Developmental Biology·James A Coffman, James M Denegre

❮ Previous
Next ❯

Related Concepts

Related Feeds

Antibody Specificity

Antibodies produced by B cells are highly specific for antigen as a result of random gene recombination and somatic hypermutation and affinity maturation. As the main effector of the humoral immune system, antibodies can neutralize foreign cells. Find the latest research on antibody specificity here.

Cell Fate Conversion By mRNA

mRNA-based technology is being studied as a potential technology that could be used to reprogram cell fate. This technique provides the potential to generate safe reprogrammed cells that can be used for clinical applications. Here is the latest research on cell fate conversion by mRNA.

Related Papers

Developmental Dynamics : an Official Publication of the American Association of Anatomists
Natalia VolodinaKimberly L Mowry
Developmental Dynamics : an Official Publication of the American Association of Anatomists
Christian SardetJanet Chenevert
© 2021 Meta ULC. All rights reserved