Localized recombination drives diversification of killing spectra for phage-derived syringacins

The ISME Journal
David A BaltrusKevin L Hockett

Abstract

To better understand the potential for antagonistic interactions between members of the same bacterial species, we have surveyed bacteriocin killing activity across a diverse suite of strains of the phytopathogen Pseudomonas syringae. Our data demonstrate that killing activity from phage-derived bacteriocins of P. syringae (R-type syringacins) is widespread. Despite a high overall diversity of bacteriocin activity, strains can broadly be classified into five main killing types and two main sensitivity types. Furthermore, we show that killing activity switches frequently between strains and that switches correlate with localized recombination of two genes that together encode the proteins that specify bacteriocin targeting. Lastly, we demonstrate that phage-derived bacteriocin killing activity can be swapped between strains simply through expression of these two genes in trans. Overall, our study characterizes extensive diversity of killing activity for phage-derived bacteriocins of P. syringae across strains and highlights the power of localized recombination to alter phenotypes that mediate strain interactions during evolution of natural populations and communities.

References

Jan 1, 1978·Canadian Journal of Microbiology·A K Vidaver, S Buckner
Jul 1, 1989·Proceedings of the National Academy of Sciences of the United States of America·I LamontJ B Egan
Jun 1, 1972·Canadian Journal of Microbiology·A K VidaverM L Schuster
Mar 2, 2002·Applied and Environmental Microbiology·Paola LavermicoccaAngelo Visconti
Jul 27, 2002·Annual Review of Microbiology·Margaret A Riley, John E Wertz
Aug 13, 2003·Bioinformatics·Fredrik Ronquist, John P Huelsenbeck
Apr 29, 2008·Applied and Environmental Microbiology·Steven R WilliamsDean Scholl
Sep 15, 2009·Current Biology : CB·Helen C LovellDawn L Arnold
Feb 2, 2010·Journal of Bacteriology·Thilo KöhlerChristian van Delden
Apr 5, 2011·The American Naturalist·Britt KoskellaAngus Buckling
Nov 18, 2011·The American Naturalist·Vanni BucciJoão B Xavier
Feb 3, 2012·Advances in Experimental Medicine and Biology·Petr G Leiman, Mikhail M Shneider
Mar 15, 2012·Molecular Plant-microbe Interactions : MPMI·David A BaltrusJeffery L Dangl
Nov 13, 2012·Ecology and Evolution·Hadas HawlenaCurtis M Lively
Dec 27, 2012·Nature Reviews. Microbiology·Paul D CotterColin Hill
Jan 15, 2014·Cell Reports·Jon PentermanPradeep K Singh
May 28, 2014·Nature Reviews. Microbiology·Dan I Andersson, Diarmaid Hughes
Aug 15, 2014·Otology & Neurotology : Official Publication of the American Otological Society, American Neurotology Society [and] European Academy of Otology and Neurotology·Lingsheng LiFrank R Lin
Oct 1, 2015·Trends in Microbiology·U G Mueller, J L Sachs
Oct 5, 2015·Trends in Microbiology·Maarten G K Ghequire, René De Mot
Nov 7, 2015·Science·Katharine Z CoyteKevin R Foster
Feb 27, 2016·Trends in Genetics : TIG·Ravi U ShethHarris H Wang
Jan 25, 2017·Journal of Visualized Experiments : JoVE·Kevin L Hockett, David A Baltrus
Apr 4, 2017·Genome Biology and Evolution·Honour C McCannHongwen Huang
Sep 30, 2017·Annual Review of Virology·Dean Scholl
Oct 19, 2017·Nature Ecology & Evolution·Britt KoskellaC Jessica E Metcalf
Feb 7, 2018·Molecular Microbiology·Nicholas M I TaylorPetr G Leiman

❮ Previous
Next ❯

Citations

Feb 21, 2019·Biochemical Society Transactions·Dorien DamsYves Briers
Oct 13, 2020·Frontiers in Microbiology·William M RooneyDaniel Walker

❮ Previous
Next ❯

Software Mentioned

tblastn
ClustalX
MLSA
R

Related Concepts

Related Feeds

Bacteriophage: Phage Therapy

Phage therapy uses bacterial viruses (bacteriophages) to treat bacterial infections and is widely being recognized as an alternative to antibiotics. Here is the latest research.