Locally-applied 5-fluorouracil-loaded slow-release patch prevents pancreatic cancer growth in an orthotopic mouse model

Oncotarget
In Kyong ShimSong Cheol Kim

Abstract

To obtain improved efficacy against pancreatic cancer, we investigated the efficacy and safety of a locally-applied 5-fluorouracil (5-FU)-loaded polymeric patch on pancreatic tumors in an orthotopic nude-mouse model. The 5-FU-releasing polymeric patch was produced by 3D printing. After application of the patch, it released the drug slowly for 4 weeks, and suppressed BxPC-3 pancreas cancer growth. Luciferase imaging of BxPC3-Luc cells implanted in the pancreas was performed longitudinally. The drug patch delivered a 30.2 times higher level of 5-FU than an intra-peritoneal (i.p.) bolus injection on day-1. High 5-FU levels were accumulated within one week by the patch. Four groups were compared for efficacy of 5-FU. Drug-free patch as a negative control (Group I); 30% 5-FU-loaded patch (4.8 mg) (Group II); 5-FU i.p. once (4.8 mg) (Group III); 5-FU i.p. once a week (1.2 mg), three times (Group IV). The tumor growth rate was significantly faster in Group I than Group II, III, IV (p=0.047 at day-8, p=0.022 at day-12, p=0.002 at day-18 and p=0.034 at day-21). All mice in Group III died of drug toxicity within two weeks after injection. Group II showed more effective suppression of tumor growth than Group IV (p=0.018 at day-12 and p=0....Continue Reading

References

Jun 15, 1992·Proceedings of the National Academy of Sciences of the United States of America·X FuR M Hoffman
Oct 2, 2003·British Journal of Cancer·M V Blagosklonny
Dec 18, 2003·Drug Discovery Today·Mikhail V Blagosklonny
Nov 1, 2005·Cell Cycle·Mikhail V Blagosklonny
Dec 17, 2005·Leukemia·M V Blagosklonny
Feb 8, 2008·Journal of Pharmaceutical Sciences·Deng Guang YuXiang Liang Yang
Sep 9, 2010·JAMA : the Journal of the American Medical Association·John P NeoptolemosUNKNOWN European Study Group for Pancreatic Cancer
Apr 11, 2013·Journal of the American Pharmacists Association : JAPhA·Iulia D UrsanAndrea Pierce
Jun 12, 2016·Journal of Controlled Release : Official Journal of the Controlled Release Society·Hee-Gyeong YiDong-Woo Cho

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Citations

Sep 1, 2018·Expert Opinion on Drug Delivery·Gilles Sebastiaan van TienderenGordon G Wallace
Feb 15, 2019·Experimental Dermatology·Xiaoping YangJohn T Seykora
Feb 14, 2021·Reproductive Toxicology·Gerile NarenBuhe Nashun
Jun 7, 2019·Nanomedicine : Nanotechnology, Biology, and Medicine·Andrew E MasseySubhash C Chauhan

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Methods Mentioned

BETA
scanning electron microscopy
xenograft
protein assay

Software Mentioned

GraphPad Prism
ImageQuant
Living Image

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