Locating the binding sites of Pb(II) ion with human and bovine serum albumins.

PloS One
A BelatikH A Tajmir-Riahi

Abstract

Lead is a potent environmental toxin that has accumulated above its natural level as a result of human activity. Pb cation shows major affinity towards protein complexation and it has been used as modulator of protein-membrane interactions. We located the binding sites of Pb(II) with human serum (HSA) and bovine serum albumins (BSA) at physiological conditions, using constant protein concentration and various Pb contents. FTIR, UV-visible, CD, fluorescence and X-ray photoelectron spectroscopic (XPS) methods were used to analyse Pb binding sites, the binding constant and the effect of metal ion complexation on HSA and BSA stability and conformations. Structural analysis showed that Pb binds strongly to HSA and BSA via hydrophilic contacts with overall binding constants of K(Pb-HSA) = 8.2 (±0.8)×10(4) M(-1) and K(Pb-BSA) = 7.5 (±0.7)×10(4) M(-1). The number of bound Pb cation per protein is 0.7 per HSA and BSA complexes. XPS located the binding sites of Pb cation with protein N and O atoms. Pb complexation alters protein conformation by a major reduction of α-helix from 57% (free HSA) to 48% (metal-complex) and 63% (free BSA) to 52% (metal-complex) inducing a partial protein destabilization.

References

Jul 16, 1992·Nature·X M He, D C Carter
Jan 1, 1986·Advances in Protein Chemistry·S Krimm, J Bandekar
Jan 1, 1985·Advances in Protein Chemistry·T Peters
Jan 1, 1985·Comparative Biochemistry and Physiology. B, Comparative Biochemistry·M S Kaldy, C L Darcel
Jan 1, 1994·Advances in Protein Chemistry·D C Carter, J X Ho
Sep 1, 1996·Toxicologic Pathology·T S Peters
Oct 24, 2002·Biochemical Pharmacology·Nicole A KratochwilPaul R Gerber
Feb 9, 2005·Bioorganic & Medicinal Chemistry·Wenying HeFenling Sheng
Jun 8, 2006·Cell Biochemistry and Biophysics·A Ahmed-OuameurH A Tajmir-Riahi
Sep 13, 2006·The Journal of Chemical Physics·Anne Bernheim-GroswasserOleg Krichevsky
Sep 25, 2007·Biomacromolecules·R BeaucheminH A Tajmir-Riahi
Dec 11, 2007·Biomacromolecules·Li LiangMuriel Subirade
Aug 8, 2008·Journal of Inorganic Biochemistry·Michael Kirberger, Jenny J Yang
Dec 5, 2008·Biological Trace Element Research·Ling Qufei, Hong Fashui
Jan 13, 2009·Journal of Pharmaceutical and Biomedical Analysis·Jean-Sébastien MandevilleH A Tajmir-Riahi
Jan 16, 2009·The Journal of Physical Chemistry. B·David CharbonneauHeidar-Ali Tajmir-Riahi
Apr 23, 2009·The Journal of Physical Chemistry. B·E FroehlichH A Tajmir-Riahi
May 29, 2009·Journal of Pharmaceutical and Biomedical Analysis·Nadim TayehJihad René Albani
Jan 21, 2010·Biomacromolecules·J S Mandeville, H A Tajmir-Riahi
May 4, 2010·Biomacromolecules·S DubeauH A Tajmir-Riahi
May 28, 2011·Journal of the American Chemical Society·Krystal A MoralesTatyana I Igumenova
Dec 27, 2011·European Journal of Medicinal Chemistry·A BelatikH A Tajmir-Riahi

❮ Previous
Next ❯

Citations

Nov 20, 2013·Spectrochimica Acta. Part A, Molecular and Biomolecular Spectroscopy·Yuandong Hu, Liangjun Da
Feb 14, 2014·Environmental Science and Pollution Research International·Yan LiuLing Song
Apr 25, 2013·Journal of Photochemistry and Photobiology. B, Biology·Ahmed BelatikRobert Carpentier
Nov 14, 2012·Journal of Photochemistry and Photobiology. B, Biology·Daniel AgudeloHeidar-Ali Tajmir-Riahi
Aug 7, 2014·The Journal of Physical Chemistry. B·Hao ZhangYadong Chen
Dec 17, 2014·The Journal of Physical Chemistry. B·Hao ZhangYadong Chen
Apr 5, 2014·Journal of Molecular Recognition : JMR·Yan LiuLing Song
Apr 29, 2014·Journal of Biochemical and Molecular Toxicology·Mingmao ChenQiqing Zhang
Dec 12, 2013·Chemical Biology & Drug Design·Mei LiHong Liang
Aug 18, 2020·International Journal of Nanomedicine·Zhong LuJin-Ming Yu
Nov 9, 2020·International Journal of Biological Macromolecules·Santosh L GawaliP A Hassan
Nov 4, 2020·Materials·Oksana KlokStefano Mischler
Mar 3, 2020·Spectrochimica Acta. Part A, Molecular and Biomolecular Spectroscopy·G A GamovV A Sharnin
Apr 14, 2021·Langmuir : the ACS Journal of Surfaces and Colloids·Aloke BapliDebabrata Seth
Jun 11, 2014·The Journal of Physical Chemistry. B·Tatiana N TikhonovaGalina P Petrova

❮ Previous
Next ❯

Methods Mentioned

BETA
X-ray
infrared spectroscopy

Software Mentioned

Jasco Standard Analysis
CDPro
CDSSTR
CasaXPS
GRAMS
Grams AI
AI

Related Concepts

Related Feeds

Cajal Bodies & Gems

Cajal bodies or coiled bodies are dense foci of coilin protein. Gemini of Cajal bodies, or gems, are microscopically similar to Cajal bodies. It is believed that Cajal bodies play important roles in RNA processing while gems assist the Cajal bodies. Find the latest research on Cajal bodies and gems here.