PMID: 7665476Sep 1, 1995Paper

Location and characteristics of the transfer region of a Bacteroides conjugative transposon and regulation of transfer genes

Journal of Bacteriology
L Y LiA A Salyers

Abstract

Many Bacteroides clinical isolates contain large conjugative transposons, which excise from the genome of a donor and transfer themselves to a recipient by a process that requires cell-to-cell contact. It has been suggested that the transfer intermediate of the conjugative transposons is a covalently closed circle, which is transferred by the same type of rolling circle mechanism used by conjugative plasmids, but the transfer origin of a conjugative transposon has not previously been localized and characterized. We have now identified the transfer origin (oriT) region of one of the Bacteroides conjugative transposons, TcrEmr DOT, and have shown that it is located near the middle of the conjugative transposon. We have also identified a 16-kbp region of the conjugal transposon which is necessary and sufficient for conjugal transfer of the element and which is located near the oriT. This same region proved to be sufficient for mobilization of coresident plasmids and unlinked integrated elements as well as for self-transfer, indicating that all of these activities are mediated by the same transfer system. Previously, we had reported that disruption of a gene, rteC, abolished self-transfer of the element. rteC is one of a set of rte...Continue Reading

Citations

Oct 1, 2013·Journal of Bacteriology·Jillian L WatersA A Salyers
Sep 22, 2016·Current Microbiology·Xiaofei JiBoqing Li
Oct 29, 1997·Proceedings of the National Academy of Sciences of the United States of America·S AgarwalMark J McBride
Apr 10, 2008·Journal of Internal Medicine·P Courvalin
Jul 17, 2014·The Journal of Antimicrobial Chemotherapy·Yaligara VeeranagoudaHannah M Wexler
Oct 8, 1999·The Journal of Antimicrobial Chemotherapy·W O ChungM Roberts
Aug 29, 2001·Clinical Infectious Diseases : an Official Publication of the Infectious Diseases Society of America·P Courvalin, P Trieu-Cuot
Nov 2, 2002·Molecular Microbiology·Vincent BurrusGérard Guédon
May 1, 2012·Applied Microbiology and Biotechnology·Xiaofei JiXuemei Lu
Apr 6, 2012·Mobile Genetic Elements·Mai Nguyen, Gayatri Vedantam
Jul 24, 2012·Journal of Bacteriology·Jillian L Waters, A A Salyers
May 15, 2012·Journal of Bacteriology·Abhishek ShrivastavaMark J McBride
Apr 6, 2016·Journal of Bacteriology·Yongtao Zhu, Mark J McBride
Jun 12, 2003·Critical Reviews in Oral Biology and Medicine : an Official Publication of the American Association of Oral Biologists·Margaret J Duncan
Aug 30, 2017·Applied Microbiology and Biotechnology·Yongtao Zhu, Mark J McBride
May 31, 2001·Microbiology and Molecular Biology Reviews : MMBR·I Chopra, M Roberts
Jun 22, 2001·Journal of Bacteriology·David W Hunnicutt, Mark J McBride
Apr 5, 2005·Journal of Bacteriology·M BacicC J Smith
Oct 4, 2005·Journal of Bacteriology·Timothy F BraunMark J McBride
Oct 19, 2000·Antimicrobial Agents and Chemotherapy·S RiedlJ Hacker
Nov 24, 2006·Journal of Bacteriology·Neil A WesslundA A Salyers
Jan 29, 2000·Journal of Bacteriology·David W Hunnicutt, Mark J McBride
Aug 30, 2008·Applied and Environmental Microbiology·Manuela Mally, Guy R Cornelis
Aug 4, 2005·Journal of Bacteriology·Kyung MoonA A Salyers
Jun 27, 2000·Journal of Bacteriology·Q ChengA A Salyers
Apr 26, 2001·Plasmid·G BonheyoA A Salyers
Jul 11, 1998·Plasmid·C J SmithD P Bayley

Related Concepts

RteB protein, Bacteriodes
Bacterial Proteins
Bacteroides
Chromosomes, Bacterial
Bacterial Conjugation
DNA Transposons
Genes, Bacterial
Episomes
Repressor Molecules
Transcription Factor

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Lipidomics & Rhinovirus Infection

Lipidomics can be used to examine the lipid species involved with pathogenic conditions, such as viral associated inflammation. Discovered the latest research on Lipidomics & Rhinovirus Infection.

Spatio-Temporal Regulation of DNA Repair

DNA repair is a complex process regulated by several different classes of enzymes, including ligases, endonucleases, and polymerases. This feed focuses on the spatial and temporal regulation that accompanies DNA damage signaling and repair enzymes and processes.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Torsion Dystonia

Torsion dystonia is a movement disorder characterized by loss of control of voluntary movements appearing as sustained muscle contractions and/or abnormal postures. Here is the latest research.

Archaeal RNA Polymerase

Archaeal RNA polymerases are most similar to eukaryotic RNA polymerase II but require the support of only two archaeal general transcription factors, TBP (TATA-box binding protein) and TFB (archaeal homologue of the eukaryotic general transcription factor TFIIB) to initiate basal transcription. Here is the latest research on archaeal RNA polymerases.

Alzheimer's Disease: MS4A

Variants within the membrane-spanning 4-domains subfamily A (MS4A) gene cluster have recently been implicated in Alzheimer's disease in genome-wide association studies. Here is the latest research on Alzheimer's disease and MS4A.

Central Pontine Myelinolysis

Central Pontine Myelinolysis is a neurologic disorder caused most frequently by rapid correction of hyponatremia and is characterized by demyelination that affects the central portion of the base of the pons. Here is the latest research on this disease.