PMID: 108098Jan 2, 1979

Location and encapsidation of the coat protein cistron of tobacco mosaic virus. A bidirectional elongation of the nucleoprotein rod

European Journal of Biochemistry
George P Lomonossoff, P J Butler

Abstract

The coat protein cistron of tobacco mosaic virus has been located on the viral RNA starting between 975 and 1050 nucleotides from the 3'-hydroxyl end. This locates its 5' end close to the origin for virus assembly, where the first protein disk interacts with RNA. It also means that the coat protein mRNA must have a short 5'-untranslated tail and a long (over 500 nucleotides) 3' one. The recovery of characteristic oligonucleotides in nuclease-protected rods during the growth from RNA and a protein disk preparation shows that elongation of the nucleated rods proceeds independently in both directions though, on average, much more rapidly along the longer 5' tail than the shorter 3' tail. Protected RNA of length equal to that in the complete virion is first seen within 6 min, showing that the most rapidly elongated particles are substantially complete by this time.

References

Jul 1, 1975·Nucleic Acids Research·D Zimmern
Dec 1, 1977·Nucleic Acids Research·M SilberklangU L RajBhandary
Jan 1, 1977·Proceedings of the National Academy of Sciences of the United States of America·G LebeurierK Richards
Apr 1, 1978·Proceedings of the National Academy of Sciences of the United States of America·M FukudaI Takebe
May 1, 1977·Proceedings of the National Academy of Sciences of the United States of America·Y OtsukiY Okada
Jan 1, 1977·Advances in Protein Chemistry·P JonathanA C Durham
Apr 29, 1976·Nature·T R HunterD Zimmern
Jan 18, 1973·Molecular & General Genetics : MGG·P J Butler, A Klug
Jan 13, 1971·Nature: New Biology·A C DurhamA Klug
Nov 1, 1974·Nucleic Acids Research·M Noll
Oct 15, 1955·Proceedings of the National Academy of Sciences of the United States of America·H FRAENKEL-CONRAT, R C Williams

Citations

Jan 1, 1979·Journal of Supramolecular Structure·K C Holmes
Jun 1, 1987·Proceedings of the National Academy of Sciences of the United States of America·M Fukuda, Y Okada
Aug 1, 1987·Journal of Biomolecular Structure & Dynamics·M KelveE Raukas
Apr 23, 1999·Philosophical Transactions of the Royal Society of London. Series B, Biological Sciences·P J Butler
Jan 1, 1981·Proceedings of the National Academy of Sciences of the United States of America·S J ShireT M Schuster
Nov 11, 1980·Biochemistry·C J Houwing, E M Jaspars
May 5, 1980·FEBS Letters·George P Lomonossoff, P J Butler
Jun 24, 2016·Beilstein Journal of Nanotechnology·Claudia KochChristina Wege

Related Concepts

Genes, Viral
RNase Pch 1
Nucleoproteins
Oligoribonucleotides
Ribonucleoproteins
RNA, Viral
Tobacco Mosaic Virus
Viral Proteins

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