Location of an F-pilin pool in the inner membrane.

Journal of Bacteriology
D MooreK Ippen-Ihler

Abstract

Polyacrylamide gel analysis of [35S]methionine-labeled membrane preparations from Escherichia coli has revealed the presence of five polypeptides present only in the membranes of cells containing the conjugative plasmid F. In addition to the previously reported product of traT, polypeptides migrating with apparent molecular weights of 100,000, 23,500, 12,000, and 7,000 were resolved. Membrane preparations from F traJ mutants lacked these polypeptides, indicating that all of these proteins are tra gene products. The 7,000-molecular-weight polypeptide comigrated with unlabeled purified F-pilin protein. About 4 to 5% of the total radioactive label in whole membrane preparations was present in this polypeptide, indicating the existence of a substantial pool of membrane-associated F-pilin. The polypeptide could be extracted from whole membrane preparations with Triton X-100 and was found in the inner membrane fraction of membranes separated by sucrose density centrifugation.

References

Nov 1, 1977·Journal of Bacteriology·H G RidgwayM I Simon
Nov 1, 1977·Proceedings of the National Academy of Sciences of the United States of America·M AchtmanR Skurray
Dec 15, 1977·Nature·N KennedyP Herrlich
Mar 1, 1978·Proceedings of the National Academy of Sciences of the United States of America·R Helmuth, M Achtman
Feb 1, 1979·Molecular & General Genetics : MGG·N Willetts, J Maule
May 1, 1979·Journal of Bacteriology·J F LutkenhausW D Donachie
Oct 1, 1979·Proceedings of the National Academy of Sciences of the United States of America·M AchtmanP Herrlich
May 5, 1977·Journal of Molecular Biology·N Willetts
Nov 11, 1976·Journal of Molecular Biology·E G MinkleyK Ippen-Ihler
Mar 1, 1974·Journal of Bacteriology·C P Novotny, P Fives-Taylor
Sep 15, 1973·Journal of Molecular Biology·F W Studier
Jan 1, 1969·Annual Review of Microbiology·R Curtiss
Jun 1, 1969·Bacteriological Reviews·D A Marvin, B Hohn
Jan 1, 1980·Journal of Bacteriology·G D ArmstrongW Paranchych
Apr 25, 1980·Journal of Molecular Biology·M AchtmanN Willetts

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Citations

Jun 15, 1984·European Journal of Biochemistry·I CalderonG C Mora
Jan 1, 1982·Molecular & General Genetics : MGG·D MooreK Ippen-Ihler
Nov 24, 1999·Molecular Microbiology·R L HarrisP M Silverman
May 18, 2010·Integrative Biology : Quantitative Biosciences From Nano to Macro·Philip M Silverman, Margaret B Clarke
Apr 1, 1986·Molecular & General Genetics : MGG·M SyvanenR Kolodner
Sep 25, 2002·Journal of Bacteriology·Michael J GubbinsLaura S Frost
Jun 4, 2014·Molecular Microbiology·Christina M BeckChristopher S Hayes
Nov 8, 2005·Microbiology·Katrin DaehnelPhilip Silverman
Jul 12, 2003·FEMS Microbiology Letters·T D LawleyL S Frost
Jan 1, 1981·Molecular & General Genetics : MGG·D MooreK Ippen-Ihler
Mar 1, 1993·Journal of Bacteriology·K ManeewannakulK Ippen-Ihler
Apr 1, 1983·Journal of Bacteriology·J McEwenP M Silverman
Mar 1, 1993·Journal of Bacteriology·D MooreK Ippen-Ihler
Jul 1, 1996·Journal of Bacteriology·N MajdalaniK Ippen-Ihler
Feb 1, 1983·Journal of Bacteriology·B A SowaK Ippen-Ihler
Oct 1, 1984·Journal of Bacteriology·D C Dodd, B I Eisenstein
Oct 1, 1984·Journal of Bacteriology·L S FrostN S Willetts
Nov 1, 1982·Journal of Bacteriology·T H WattsW Paranchych
Jul 1, 1987·Journal of Bacteriology·K A SchandelR E Webster
Dec 1, 1991·Journal of Bacteriology·L S Frost, D P Bazett-Jones
Jun 1, 1994·Microbiological Reviews·L S FrostR A Skurray
Jan 1, 1982·Developmental Biology·J Smit, N Agabian

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