PMID: 3754927Jan 1, 1986Paper

Location of gene expression in CNS using hybridization histochemistry

Methods in Enzymology
J PenschowJ Coghlan

Abstract

In this chapter we have placed heavy emphasis on our own recent work to lay out a workable recipe for hybridization histochemistry. Only a trickle of papers followed the initial benchmark excursions into in situ labeling of tissue sections. Our own entry into this field was as late starters in 1978, but since then a confluence of important questions and technical advances has served to make hybridization histochemistry much more attractive and universally applicable as a research tool. Hybridization histochemistry allows the location of anatomical sites of gene expression and viral replication with unique specificity and is able to solve some problems for which there is no other suitable technique available in the central nervous system. For example, allowing that peptides may enter neurons by a variety of mechanisms and then be christened neuroendocrine peptides, it has become a compelling issue to know which cells are manufacturing the peptide. Thus, much can be learned by the approach elegantly demonstrated by Gee et al., of locating mRNA and its peptide product within the same neuron. The intracellular location of specific mRNA for a neuropeptide in a cell body indicates a very high probability that the peptide is secreted ...Continue Reading

Citations

Jan 1, 1988·European Journal of Immunology·P F Gallagher, J F Miller
Nov 11, 1998·Clinical and Experimental Pharmacology & Physiology. Supplement·A Butkus, J P Coghlan
Jul 15, 1987·Experientia·J D PenschowJ P Coghlan
Sep 1, 1994·Journal of Oral Pathology & Medicine : Official Publication of the International Association of Oral Pathologists and the American Academy of Oral Pathology·K YamazakiK Hara
Jun 1, 1990·AIDS Research and Human Retroviruses·R KiernanD McPhee
May 1, 1993·Microscopy Research and Technique·P M WiseA Cai
Oct 1, 1991·Molecular and Cellular Endocrinology·J D PenschowJ P Coghlan
Nov 1, 1991·Molecular and Cellular Endocrinology·M C KeightleyP J Fuller

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