PMID: 11310434Apr 20, 2001Paper

Location of the initiation site of calcium transients and sparks in rabbit heart Purkinje cells

The Journal of Physiology
J M CordeiroJ H Bridge

Abstract

1. The distribution and localization of Ca2+ transients and Ca2+ sparks in isolated adult rabbit Purkinje cells were examined using confocal microscopy and the Ca2+ indicator fluo-3. 2. When cells were field stimulated in 2.0 mM Ca2+ buffer, a transverse confocal line scan (500 Hz) showed that the fluorescence intensity was greatest at the cell periphery during the onset of the Ca2+ transient ([Ca2+]i). In contrast, the [Ca2+]i of ventricular cells showed a more uniform pattern of activation across the cell. Staining with di-8-ANEPPS revealed that Purkinje cells lack t-tubules, whereas ventricular cells have an extensive t-tubular system. 3. When we superfused both cell types with a buffer containing 5 mM Ca2+-1 microM isoproterenol (isoprenaline) they produced Ca2+ sparks spontaneously. Ca2+ sparks occurred only at the periphery of Purkinje cells but occurred throughout ventricular cells. Sparks in both cell types could be completely abolished by addition of the SR inhibitor thapsigargin (500 nM). Brief exposure to nifedipine (10 microM) did not reduce the number of spontaneous sparks. 4. Immunofluorescence staining of Purkinje cells with anti-ryanodine antibody revealed that ryanodine receptors (RyRs) are present at both peri...Continue Reading

References

Aug 1, 1992·The American Journal of Physiology·F A LaiG Meissner
Aug 1, 1992·Biophysical Journal·M D Stern
Dec 1, 1989·The American Journal of Physiology·F Scamps, E Carmeliet
Sep 8, 1965·Annals of the New York Academy of Sciences·R C Truex, M Q Smythe
Mar 1, 1968·The Journal of Cell Biology·J R Sommer, E A Johnson
Jan 1, 1982·Pflügers Archiv : European journal of physiology·W G Wier, G Isenberg
Oct 1, 1994·Pflügers Archiv : European journal of physiology·H ChengW J Lederer
Sep 15, 1995·The Journal of Physiology·P S ShacklockC W Balke
Jan 1, 1996·The American Journal of Physiology·H ChengM B Cannell
Aug 1, 1996·The Journal of Physiology·J HüserL A Blatter
Jun 20, 1998·The Journal of Physiology·J M CordeiroW R Giles
Jun 25, 1999·The Journal of Physiology·J H BridgeM B Cannell

❮ Previous
Next ❯

Citations

Dec 13, 2003·Bulletin of Mathematical Biology·Greg Lemon
Nov 7, 2008·Microscopy and Microanalysis : the Official Journal of Microscopy Society of America, Microbeam Analysis Society, Microscopical Society of Canada·John A Scherschel, Michael Rubart
May 17, 2013·Proceedings of the National Academy of Sciences of the United States of America·Pawel SwietachRichard D Vaughan-Jones
Feb 15, 2012·The Journal of General Physiology·Vyacheslav M ShkrylEduardo Ríos
Feb 18, 2003·The Journal of Physiology·Marcello Rota, Mario Vassalle
Sep 6, 2011·American Journal of Physiology. Heart and Circulatory Physiology·Lu-Feng ChengAnatoli N Lopatin
Oct 2, 2007·American Journal of Physiology. Heart and Circulatory Physiology·Jonathan M CordeiroJ Andrew Wasserstrom
Feb 26, 2010·Circulation. Cardiovascular Genetics·Eyal NofCharles Antzelevitch
May 3, 2013·PloS One·Vyacheslav M Shkryl, Lothar A Blatter
Dec 29, 2009·Frontiers in Bioscience (Landmark Edition)·Pawel SwietachRichard D Vaughan-Jones
Mar 14, 2002·Proceedings of the National Academy of Sciences of the United States of America·Sebastian K G MaierWilliam A Catterall
Feb 18, 2006·The Journal of Physiology·Katherine A SheehanLothar A Blatter
May 28, 2005·The Journal of Physiology·Pawel Swietach, Richard D Vaughan-Jones
Nov 26, 2013·Archivos de cardiología de México·Héctor Barajas-MartínezCharles Antzelevitch
Nov 10, 2015·The Journal of Physiology·Thomas HofRomain Guinamard
Oct 23, 2012·Heart Rhythm : the Official Journal of the Heart Rhythm Society·Jonathan M Cordeiro
Jul 5, 2008·Journal of Molecular and Cellular Cardiology·Thomas J HundPeter J Mohler
May 13, 2008·Journal of Molecular and Cellular Cardiology·Rose M SnopkoRafael Mejía-Alvarez
Oct 4, 2007·Progress in Biophysics and Molecular Biology·K H W J Ten Tusscher, A V Panfilov
Dec 23, 2006·Journal of Molecular and Cellular Cardiology·Robert Dumaine, Jonathan M Cordeiro
Aug 23, 2005·Biophysical Journal·Masashi Inoue, John H B Bridge
Aug 14, 2012·Progress in Biophysics and Molecular Biology·Thomas G McNaryFrank B Sachse
Feb 8, 2011·Biochimica Et Biophysica Acta·Martin D BootmanH Llewelyn Roderick
May 27, 2015·The Journal of General Physiology·Pavol PetrovičAlexandra Zahradníková
Jul 20, 2011·Journal of Molecular and Cellular Cardiology·Eric A Sobie, W J Lederer
Apr 11, 2006·Biophysical Journal·Ye Chen-IzuLeighton T Izu
Apr 9, 2008·Biophysical Journal·Pawel SwietachRichard D Vaughan-Jones
Dec 19, 2014·Frontiers in Physiology·Xiao-Long Lu, Michael Rubart
Jan 15, 2011·Journal of Molecular and Cellular Cardiology·Young-Seon LeeEric A Sobie
Apr 10, 2003·Science's STKE : Signal Transduction Knowledge Environment·Donald M Bers

❮ Previous
Next ❯

Related Concepts

Related Feeds

Cardiac Electrophysiology

Cardiac electrophysiology is the study of electrical activities of the heart and includes the assessment, diagnosis, and treatment of cardiac events. Find the latest research on cardiac electrophysiology here.