Locomotion Induced by Spatial Restriction in Adult Drosophila

PloS One
Chengfeng Xiao, R Meldrum Robertson

Abstract

Drosophila adults display an unwillingness to enter confined spaces but the behaviors induced by spatial restriction in Drosophila are largely unknown. We developed a protocol for high-throughput analysis of locomotion and characterized features of locomotion in a restricted space. We observed intense and persistent locomotion of flies in small circular arenas (diameter 1.27 cm), whereas locomotion was greatly reduced in large circular arenas (diameter 3.81 cm). The increased locomotion induced by spatial restriction was seen in male flies but not female flies, indicating sexual dimorphism of the response to spatial restriction. In large arenas, male flies increased locomotion in arenas previously occupied by male but not female individuals. In small arenas, such pre-conditioning had no effect on male flies, which showed intense and persistent locomotion similar to that seen in fresh arenas. During locomotion with spatial restriction, wildtype Canton-S males traveled slower and with less variation in speed than the mutant w1118 carrying a null allele of white gene. In addition, wildtype flies showed a stronger preference for the boundary than the mutant in small arenas. Genetic analysis with a series of crosses revealed that th...Continue Reading

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Citations

Jan 15, 2017·Comparative Biochemistry and Physiology. Part A, Molecular & Integrative Physiology·Shuang QiuR Meldrum Robertson
Jul 10, 2019·The Journal of Experimental Biology·Chengfeng XiaoAdam K Chippindale
Apr 1, 2016·Genetics·Chengfeng Xiao, R Meldrum Robertson
Aug 11, 2017·Scientific Reports·Chengfeng XiaoR Meldrum Robertson
Nov 4, 2017·Behavior Genetics·Chengfeng XiaoR Meldrum Robertson
Nov 14, 2020·Zoology : Analysis of Complex Systems, ZACS·Shuang QiuChengfeng Xiao
Mar 6, 2019·The Science of the Total Environment·Shuang QiuShijian Ge

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