May 14, 2014

Iron acquisition system of Sphingobium sp. strain SYK-6, a degrader of lignin-derived aromatic compounds

BioRxiv : the Preprint Server for Biology
Tara Lydiard-MartinEiji Masai

Abstract

Iron, an essential element for all organisms, acts as a cofactor of enzymes in bacterial degradation of recalcitrant aromatic compounds. The bacterial family, Sphingomonadaceae comprises various degraders of recalcitrant aromatic compounds; however, little is known about their iron acquisition system. Here, we investigated the iron acquisition system in a model bacterium capable of degrading lignin-derived aromatics, Sphingobium sp. strain SYK-6. Analyses of SYK-6 mutants revealed that FiuA (SLG\_34550), a TonB-dependent receptor (TBDR), was the major outer membrane iron transporter. Three other TBDRs encoded by SLG\_04340, SLG\_04380, and SLG\_10860 also participated in iron uptake, and tonB2 (SLG\_34550), one of the six tonB comprising the Ton complex which enables TBDR-mediated transport was critical for iron uptake. The ferrous iron transporter FeoB (SLG\_36840) played an important role in iron uptake across the inner membrane. The promoter activities of most of the iron uptake genes were induced under iron-limited conditions, and their regulation is controlled by SLG_29410 encoding the ferric uptake regulator, Fur. Although feoB , among all the iron uptake genes identified is highly conserved in Sphingomonad strains, the o...Continue Reading

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Mentioned in this Paper

Embryo
Embryo Structure
Genome
Genes
Locus Ceruleus Morphogenesis
Hsc70-4
Gene Deletion
Promoter
Drosophila
Plants, Transgenic

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