LOMIX, a Mixture of Flaxseed Linusorbs, Exerts Anti-Inflammatory Effects through Src and Syk in the NF-κB Pathway.

Biomolecules
Zubair Ahmed RatanJae Youl Cho

Abstract

Although flax (Linum usitatissimum L.) has long been used as Ayurvedic medicine, its anti-inflammatory role is still unclear. Therefore, we aimed to investigate the anti-inflammatory role of a linusorb mixture (LOMIX) recovered from flaxseed oil. Effects of LOMIX on inflammation and its mechanism of action were examined using several in vitro assays (i.e., NO production, real-time PCR analysis, luciferase-reporter assay, Western blot analysis, and kinase assay) and in vivo analysis with animal inflammation models as well as acute toxicity test. Results: LOMIX inhibited NO production, cell shape change, and inflammatory gene expression in stimulated RAW264.7 cells through direct targeting of Src and Syk in the NF-κB pathway. In vivo study further showed that LOMIX alleviated symptoms of gastritis, colitis, and hepatitis in murine model systems. In accordance with in vitro results, the in vivo anti-inflammatory effects were mediated by inhibition of Src and Syk. LOMIX was neither cytotoxic nor did it cause acute toxicity in mice. In addition, it was found that LOB3, LOB2, and LOA2 are active components included in LOMIX, as assessed by NO assay. These in vitro and in vivo results suggest that LOMIX exerts an anti-inflammatory eff...Continue Reading

References

Oct 1, 1994·Toxicology and Applied Pharmacology·G B CorcoranR Buttyan
Aug 7, 1998·Clinica Chimica Acta; International Journal of Clinical Chemistry·I GuevaraA Szczudlik
Feb 28, 2002·Annual Review of Immunology·Charles A Janeway, Ruslan Medzhitov
Feb 3, 2005·CMAJ : Canadian Medical Association Journal = Journal De L'Association Medicale Canadienne·Edoardo G GianniniVincenzo Savarino
Mar 23, 2010·Cell·Osamu Takeuchi, Shizuo Akira
Jan 7, 2011·Molecular and Cellular Biochemistry·Mohammad Shamsul OlaHaseeb Ahsan
Dec 5, 2012·Mediators of Inflammation·Se Eun ByeonJae Youl Cho
Jan 11, 2013·Mediators of Inflammation·Tao YuJae Youl Cho
Oct 9, 2013·Proceedings of the National Academy of Sciences of the United States of America·Frances Y McWhorterWendy F Liu
Dec 27, 2013·The New England Journal of Medicine·William Bernal, Julia Wendon
Feb 11, 2014·Current Protocols in Immunology·Benoit ChassaingMatam Vijay-Kumar
Jul 22, 2014·Mediators of Inflammation·Young-Su YiJae Youl Cho
Mar 19, 2015·Journal of Natural Products·Youn Young ShimMartin J T Reaney
Sep 2, 2015·Nature Reviews. Gastroenterology & Hepatology·Gilaad G Kaplan
Mar 1, 2017·Lancet·Angel Lanas, Francis K L Chan
Dec 22, 2017·Differentiation; Research in Biological Diversity·René Fernando Abarca-BuisEdgar Krötzsch
Jan 20, 2018·Journal of Ginseng Research·Yo Han HongJae Youl Cho
May 5, 2018·Evidence-based Complementary and Alternative Medicine : ECAM·Han Gyung KimJae Youl Cho
Oct 20, 2018·Journal of Ginseng Research·Sang Yun HanJae Youl Cho
Dec 7, 2018·International Journal of Molecular Sciences·Qingjie XueHuabao Xiong
May 28, 2019·Nutrients·Mihir ParikhGrant N Pierce
Jun 13, 2019·Evidence-based Complementary and Alternative Medicine : ECAM·Sang Hee ParkJae Youl Cho
Oct 31, 2019·Trends in Immunology·Yansong XueSi Ming Man
Nov 14, 2019·Immune Network·Ferdaus Mohd Altaf HossainSeong Kug Eo
Jan 14, 2020·Evidence-based Complementary and Alternative Medicine : ECAM·Akash AhujaJae Youl Cho

❮ Previous
Next ❯

Citations


❮ Previous
Next ❯

Methods Mentioned

BETA
ELISA
PCR
profiler
nuclear translocation

Software Mentioned

ImageJ
LOMIX
SPSS

Related Concepts

Related Feeds

Atrophic Gastritis

Atrophic Gastritis is a process where gastric glandular cells are lost and replaced with firbous tissues, as a result of chronic inflammation. Learn more about Atrophic Gastritis here.

Related Papers

Evidence-based Complementary and Alternative Medicine : ECAM
Nurinanda Prisky QomaladewiJae Youl Cho
Inflammation & Allergy Drug Targets
Michael P SandersonChung-Wai Chow
The Korean Journal of Physiology & Pharmacology : Official Journal of the Korean Physiological Society and the Korean Society of Pharmacology
Yong KimJae Youl Cho
© 2022 Meta ULC. All rights reserved