Long- and short-range structural characteristics of pea starch modified by autoclaving, α-amylolysis, and pullulanase debranching

International Journal of Biological Macromolecules
Zhen MaJoyce I Boye

Abstract

Pea starch (S) was modified by autoclaving (A), α-amylolysis (E), and pullulanase debranching (P), the effect of pretreatments including autoclaving and α-amylolysis on the structural modifications to the pullulanase debranched starch was investigated. All processed pea starch was transformed from a C- to a B-type crystalline structure. The power law exponent (α) ranging from 1.85 to 2.64 suggested the existence of mass fractal structure. Compared with native starch, all treatments applied caused an enhanced short-range order which was reflected by the increased values of α, degree of double helix (DD), degree of order (DO), and double helix content based on SAXS, FTIR, and 13CNMR observations. The processed starch sample of AS, and APS exhibited the highest DO, and α values, as well as the stronger absorption peak between 3000 and 3695 cm-1on FT-IR spectrum. AEPS exhibited the significantly highest double helix content, indicating that the higher extent of degradation induced by the combined treatments of autoclaving, α-amylolysis, and pullulanase debranching would give the molecular chains a higher alignment opportunity for the evolution towards coil-to-helix transition. The results would be helpful for better understanding t...Continue Reading

References

Oct 10, 2012·International Journal of Biological Macromolecules·Huanxin ZhangZhengyu Jin
Feb 13, 2013·Carbohydrate Polymers·M JoshiB Adhikari
Jan 11, 2018·International Journal of Biological Macromolecules·Hilal Demirkesen-BicakDilara Nilufer-Erdil

❮ Previous
Next ❯

Related Concepts

Related Feeds

Cajal Bodies & Gems

Cajal bodies or coiled bodies are dense foci of coilin protein. Gemini of Cajal bodies, or gems, are microscopically similar to Cajal bodies. It is believed that Cajal bodies play important roles in RNA processing while gems assist the Cajal bodies. Find the latest research on Cajal bodies and gems here.