PMID: 698244Sep 28, 1978Paper

Long-chain acyl-CoA synthetase and "outer" carnitine long-chain acyltransferase activities of intact brown adipose tissue mitochondria

Biochimica Et Biophysica Acta
P T Normann, T Flatmark

Abstract

1. The activities of long-chain acyl-CoA synthetase (acid: CoA ligase (AMP-forming), EC 6.2.1.3) and the "outer" carnitine long-chain acyltransferase (palmitoyl-CoA: L-carnitine O-palmitoyltransferase, EC 2.3.1.21) have been estimated in intact brown adipose tissue mitochondria. The assay of both enzymes is based on a coupled reaction in which the intramitochondrial (matrix) CoASH is the final acyl acceptor and the oxidation-reduction state of the flavoproteins in the acyl-CoA dehydrogens pathway is used to determine the intramitochondrial level of acyl-CoA. 2. Using endogenous fatty acids as the substrate, the progress curve of acyl-CoA synthetase activity was in most mitochondrial preparations linear within the first 30 s. When initial rates were measured, the Km value for CoASH (2.4 micron) was lower than previously determined for the acyl-CoA synthetase in brown adipose tissue mitochondria as well as in mitochondria of other tissues. The pH activity curve indicates that the unprotonated form of the fatty acids represents the substrate of acyl-CoA synthetase, i.e. similar to the effect of pH on the binding of fatty acids to bovine serum albumin. 3. Experimental evidence is presented that at temperatures higher than the trans...Continue Reading

References

Jan 1, 1976·Advances in Lipid Research·P H GrootW C Hülsmann
Jul 22, 1975·Biochimica Et Biophysica Acta·J I PedersenM Aas
Mar 31, 1975·Biochimica Et Biophysica Acta·T Flatmark, J I Pedersen
Oct 1, 1975·The Biochemical Journal·K A Lloyd-Davies, D N Brindley
Jan 31, 1972·European Journal of Biochemistry·J BremerS Skrede
Jul 1, 1970·European Journal of Biochemistry·S Skrede, J Bremer
Sep 1, 1970·The Biochemical Journal·P B GarlandB A Haddock

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Citations

Jun 2, 1979·Biochimica Et Biophysica Acta·O TerlandH Kryvi
Jan 30, 2002·Progress in Lipid Research·Simon Eaton
Jul 3, 1979·Biochimica Et Biophysica Acta·D G Nicholls

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