Long-chain fatty acid activates hepatocytes through CD36 mediated oxidative stress

Lipids in Health and Disease
Jun LiuYaxi Chen

Abstract

Accumulating evidence suggests that activated hepatocytes are involved in the deposition of the excess extracellular matrix during liver fibrosis via the epithelial to mesenchymal transition. Lipid accumulation in hepatocytes are implicated in the pathogenesis of chronic liver injury. CD36 is known to mediate long-chain fatty acid (LCFA) uptake and lipid metabolism. However, it is unclear whether LCFA directly promotes hepatocyte activation and the involved mechanisms have not been fully clarified. Mice were fed with a high fat diet (HFD) and normal hepatocyte cells (Chang liver cells) were treated with palmitic acid (PA) in vivo and in vitro. Real-time polymerase chain reaction (RT-PCR) and western blotting were used to examine the gene and protein expression of molecules involved in hepatic fibrogenesis and hepatocyte activation. CD36 was knocked down by transfecting CD36 siRNA into hepatocyte cells. Hydrogen peroxide (H2O2) and reactive oxygen species (ROS) levels were detected using commercial kits. HFD induced a profibrogenic response and up-regulated CD36 expression in vivo. Analogously, PA increased lipid accumulation and induced human hepatocyte activation in vitro, which was also accompanied by increased CD36 expressio...Continue Reading

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Citations

Dec 18, 2018·IUBMB Life·Jean-Marc Zingg
Jul 12, 2019·Arteriosclerosis, Thrombosis, and Vascular Biology·Yuanyuan LiYanqiao Zhang
Nov 20, 2020·Cardiovascular Research·Hongyang ShuDao Wen Wang
Jun 16, 2021·Free Radical Biology & Medicine·Alfredo Cruz-GregorioFrancisco Mendoza-Hoffmann
Apr 2, 2020·Ecotoxicology and Environmental Safety·Ruifen KangChunmei Li

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Methods Mentioned

BETA
Protein Assay
PCR
MDA
transfection

Software Mentioned

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