Long-chain fatty acids suppress the induction of urea cycle enzyme genes by glucocorticoid action

FEBS Letters
M TomomuraT Saheki

Abstract

In order to test the possibility that free fatty acids are the mediator of the abnormal expression of urea cycle enzyme genes in carnitine-deficient juvenile visceral steatosis (JVS) mice, the effects of fatty acids on urea cycle enzyme, carbamoylphosphate synthetase (CPS) and argininosuccinate synthetase (ASS), mRNA levels were examined in rat primary cultured hepatocytes. Addition of a synthetic glucocorticoid hormone, dexamethasone, caused increases in CPS and ASS mRNAs. Further addition of oleic acid suppressed the induction of CPS and ASS mRNAs by dexamethasone. In contrast, the phosphoenolpyruvate carboxykinase (PEPCK) mRNA level induced by dexamethasone was enhanced in the presence of oleic acid. The effects were reversed on further addition of carnitine. The mRNA levels of these enzymes induced by dibutyryl cAMP were not affected by the addition of oleic acid. A study of the specificity of fatty acids revealed that long-chain fatty acids of more than 16 carbons chain length had a suppressive effect on the CPS mRNA level induced by dexamethasone and that the presence of double bonds enhanced the effect. The changes in gene expression of CPS, ASS and PEPCK caused by the fatty acids in the cultured hepatocytes were very si...Continue Reading

References

Nov 2, 1976·Biochimica Et Biophysica Acta·R Z Christiansen, J Bremer
Feb 14, 1992·Biochimica Et Biophysica Acta·M TomomuraT Saheki
Nov 1, 1991·Trends in Genetics : TIG·R Schüle, R M Evans
Sep 1, 1958·American Journal of Clinical Pathology·P G CABAUD, F WROBLEWSKI

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Citations

May 17, 2003·Metabolic Engineering·Christina ChanMartin L Yarmush
Apr 24, 2003·European Journal of Biochemistry·Annie HussonAlain Lavoinne
Jun 11, 2002·Annual Review of Nutrition·Sidney M Morris
Apr 26, 2008·Molecular BioSystems·Aram AdourianIna Schuppe-Koistinen
Feb 26, 2011·Neuroscience and Biobehavioral Reviews·Canteros Maria Griselda
May 8, 2014·Liver Transplantation : Official Publication of the American Association for the Study of Liver Diseases and the International Liver Transplantation Society·Nir I NativMartin L Yarmush
Sep 5, 2001·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·S KerstenW Wahli
Aug 7, 2019·Liver International : Official Journal of the International Association for the Study of the Liver·Peter Lykke EriksenKaren Louise Thomsen

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