Long-distance base pairing in flock house virus RNA1 regulates subgenomic RNA3 synthesis and RNA2 replication

Journal of Virology
Brett D LindenbachPaul Ahlquist

Abstract

Replication of flock house virus (FHV) RNA1 and production of subgenomic RNA3 in the yeast Saccharomyces cerevisiae provide a useful tool for the dissection of FHV molecular biology and host-encoded functions involved in RNA replication. The replication template activity of RNA1 can be separated from its coding potential by supplying the RNA1-encoded replication factor protein A in trans. We constructed a trans-replication system in yeast to examine cis-acting elements in RNA1 that control RNA3 production, as well as RNA1 and RNA2 replication. Two cis elements controlling RNA3 production were found. A proximal subgenomic control element was located just upstream of the RNA3 start site (nucleotides [nt] 2282 to 2777). A short distal element also controlling RNA3 production (distal subgenomic control element) was identified 1.5 kb upstream, at nt 1229 to 1239. Base pairing between these distal and proximal elements was shown to be essential for RNA3 production by covariation analysis and in vivo selection of RNA3-expressing replicons from plasmid libraries containing random sequences in the distal element. Two distinct RNA1 replication elements (RE) were mapped within the 3' quarter of RNA1: the intRE (nt 2322 to 2501) and the 3'...Continue Reading

References

Jan 2, 1992·Gene·T W ChristiansonP Hieter
Jan 1, 1992·Current Genetics·D C ChenT T Kuo
Jan 1, 1990·Proceedings of the National Academy of Sciences of the United States of America·B H SellingP Kaesberg
Oct 1, 1989·Proceedings of the National Academy of Sciences of the United States of America·J A JaegerM Zuker
Jan 1, 1986·Proceedings of the National Academy of Sciences of the United States of America·B DasmahapatraP Kaesberg
Dec 1, 1986·Molecular & General Genetics : MGG·D J MeadS G Oliver
Mar 20, 1985·Journal of Molecular Biology·B DasmahapatraP Kaesberg
Aug 1, 1984·Molecular and Cellular Biology·M Johnston, R W Davis
Dec 15, 1981·Journal of Molecular Biology·F MeyerC Weissmann
Jan 1, 1994·Archives of Virology. Supplementum·L A BallY Li
Dec 20, 1994·Proceedings of the National Academy of Sciences of the United States of America·L A Ball
Jun 1, 1993·Journal of Virology·L A Ball, Y Li
Sep 3, 1996·Proceedings of the National Academy of Sciences of the United States of America·B D PriceP Ahlquist
Sep 29, 1999·Proceedings of the National Academy of Sciences of the United States of America·P E LobertT Michiels
Oct 16, 1999·Proceedings of the National Academy of Sciences of the United States of America·G van MarleE J Snijder
Jul 13, 2000·Virology·W A Miller, G Koev
Jun 20, 2001·Journal of Virology·A A KhromykhE G Westaway

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Citations

Jun 3, 2008·Cellular and Molecular Life Sciences : CMLS·P A Venter, A Schneemann
Jun 29, 2010·Archives of Virology·Naomi HataToshihiro Nakai
Jul 1, 2011·Journal of Virology·Pedro A Mateos-GómezIsabel Sola
Jan 16, 2009·Journal of Virology·Priscilla M Van Wynsberghe, Paul Ahlquist
Feb 16, 2007·Journal of Virology·Priscilla M Van WynsberghePaul Ahlquist
Apr 14, 2005·Journal of Virology·Jessica E PetrilloLee Gehrke
Aug 5, 2005·Annual Review of Phytopathology·John F Bol
May 18, 2006·Annual Review of Phytopathology·W Allen Miller, K Andrew White
Apr 23, 2014·PloS One·Linhui HaoPaul Ahlquist
May 30, 2013·PLoS Pathogens·Baodong WuK Andrew White
Jun 17, 2014·Nature Reviews. Microbiology·Beth L Nicholson, K Andrew White
Feb 8, 2003·Proceedings of the National Academy of Sciences of the United States of America·Yutaka FujiiYoshihiro Kawaoka
Oct 16, 2014·International Journal of Molecular Sciences·Payal D MaharajAlison A McCormick
Sep 29, 2009·Biochimica Et Biophysica Acta·Ying LiuAniko V Paul
Mar 21, 2009·Trends in Microbiology·Ronald P van Rij, Eugene Berezikov
Mar 8, 2011·Virology·Joanna Sztuba-SolińskaJozef J Bujarski
Sep 29, 2007·Virology·David G Nickens, Richard W Hardy
Nov 30, 2014·Molecular Biotechnology·Yiyang ZhouChristopher M Kearney

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