Long-distance movement of cherry leaf roll virus in infected tobacco plants

The Journal of General Virology
P Más, V Pallas

Abstract

The long-distance movement of cherry leaf roll virus (CLRV) in tobacco plants was studied using a tissue printing technique with non-isotopic RNA probes. Time-course analysis revealed that CLRV RNA accumulated in the inoculated leaf at an early stage, such as 20 h post-inoculation. The virus accumulation reached a peak at 8-10 days post-inoculation (d.p.i.) and then progressively decreased. The virus RNA signal was detected before the appearance of symptoms. The virus invaded stem vascular tissues at 3 d.p.i., moving towards the roots before moving to the upper leaves. In systemically infected leaves, the virus appeared first in the basal regions and then moved to the distal parts through the vascular system. The distribution pattern of the virus coat protein in systemically infected leaves was parallel to that observed for the virus RNA, suggesting that CLRV requires the coat protein for long-distance movement. The movement of the virus was influenced by the phyllotactic position of the leaves. The viral symptoms and the virus RNA signal in older systemically infected leaves were asymmetrically distributed, being localized in the side of the lamina closest to the inoculated leaf. Virus distribution in infected plants as well a...Continue Reading

Citations

Mar 16, 2000·Plant Science : an International Journal of Experimental Plant Biology·P MásV Pallás
Aug 6, 2000·The Plant Journal : for Cell and Molecular Biology·N H ChengR S Nelson
Oct 26, 2000·Proceedings of the National Academy of Sciences of the United States of America·P Mas, R N Beachy
Jun 23, 2011·Phytopathology·P KogovšekM Pompe-Novak
Aug 19, 2008·Molecular Plant Pathology·Blanca Gosalvez-BernalM Amelia Sanchez-Pina
Mar 12, 2015·Molecular Plant-microbe Interactions : MPMI·Rupar MatevzRavnikar Maja
Oct 7, 2010·Molecular Plant-microbe Interactions : MPMI·Marco A García-Neria, Rafael F Rivera-Bustamante
Aug 23, 2020·Plants·Roberta SpanòTiziana Mascia

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