Long-distance transport of L-ascorbic acid in potato

BMC Plant Biology
Luigi TedoneRoberto Viola

Abstract

Following on from recent advances in plant AsA biosynthesis there is increasing interest in elucidating the factors contributing to the L-ascorbic acid (AsA) content of edible crops. One main objective is to establish whether in sink organs such as fruits and tubers, AsA is synthesised in situ from imported photoassimilates or synthesised in source tissues and translocated via the phloem. In the current work we test the hypothesis that long-distance transport is involved in AsA accumulation within the potato tuber, the most significant source of AsA in the European diet. Using the EDTA exudation technique we confirm the presence of AsA in the phloem of potato plants and demonstrate a correlation between changes in the AsA content of source leaves and that of phloem exudates. Comparison of carboxyflourescein and AgNO3 staining is suggestive of symplastic unloading of AsA in developing tubers. This hypothesis was further supported by the changes in AsA distribution during tuber development which closely resembled those of imported photoassimilates. Manipulation of leaf AsA content by supply of precursors to source leaves resulted in increased AsA content of developing tubers. Our data provide strong support to the hypothesis that...Continue Reading

References

Jan 1, 1997·Free Radical Biology & Medicine·A NandiI B Chatterjee
Jun 10, 1998·Nature·G L WheelerN Smirnoff
Mar 31, 1999·Proceedings of the National Academy of Sciences of the United States of America·P L ConklinR L Last
May 20, 1999·Nature·H TsukaguchiM A Hediger
May 10, 2000·FEMS Microbiology Letters·R D HancockR Viola
May 22, 2002·Lancet·C David Naylor
Jun 18, 2002·Plant Physiology·Georg JanderRobert L Last
Oct 12, 2002·Plant Physiology·Vincent R Franceschi, Nathan M Tarlyn
Jan 14, 2003·Nature Biotechnology·Fernanda AgiusVictoriano Valpuesta
Sep 5, 2003·The Journal of Biological Chemistry·Beata A Wolucka, Marc Van Montagu
Nov 25, 2003·BMC Plant Biology·Robert D HancockRoberto Viola
Feb 21, 2004·Plant Physiology·Argelia LorenceCraig L Nessler
Apr 10, 2004·Arteriosclerosis, Thrombosis, and Vascular Biology·Chiang-Ting ChienSu-Ming Hsu
Apr 15, 2004·Plant Molecular Biology·Jessica A RadzioCraig L Nessler
Jan 1, 1933·Plant Physiology·A S Crafts
Jan 1, 1974·Plant Physiology·R W King, J A Zeevaart

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Citations

Oct 31, 2012·Journal of Experimental Botany·Noé GestRebecca Stevens
Feb 13, 2015·Critical Reviews in Food Science and Nutrition·Christie LovatDanielle J Donnelly
Oct 6, 2006·Phytochemical Analysis : PCA·Paul G WalkerRobert D Hancock
Nov 9, 2019·Antioxidants & Redox Signaling·Christine H FoyerRobert D Hancock
Jun 10, 2011·Journal of the Science of Food and Agriculture·Vanessa J MelinoChristopher M Ford
May 5, 2018·Journal of Experimental Botany·William PlumbChristine H Foyer
Jul 13, 2018·Frontiers in Molecular Biosciences·Vito ScaleraAnnamaria Tonazzi
Dec 19, 2019·Antioxidants·Karolina BilskaEwa Marzena Kalemba
Mar 24, 2016·Plant Molecular Biology·Valentino RuggieriMaria Luisa Chiusano
Jun 15, 2010·Comptes rendus biologies·Sylvie DinantJulia Kehr

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Methods Mentioned

BETA
AsA
transgenic

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