Abstract
RNA interference (RNAi) refers to the selective degradation of mRNA induced by double-stranded RNA (dsRNA), first discovered in Caenorhabditis elegans. Homology-dependent silencing phenomena related to RNAi have been observed in many species from all eukaryotic kingdoms. RNAi and related mechanisms share several conserved components. The hallmark of these phenomena is the presence of short dsRNA molecules (21-25 bp long), termed short interfering RNA (siRNA), which are generated from dsRNA by the activity of Dicer, a specific type III RNAse. These molecules serve as a template for the recognition and cleavage of the cognate mRNA. As it is beyond the scope of a single review to cover all aspects of RNAi, this review will focus on certain steps of the pathway relevant to mammals and on the use of long dsRNA to specifically silence genes in mammalian cells permissive to this technique, such as oocytes and early embryos.
Citations
May 13, 2009·Cell Research·Xinzheng Guo, Shaorong Gao
Dec 5, 2008·Human Reproduction·X Wu
Jan 9, 2009·Biology of Reproduction·Ulyana VjuginaJanice P Evans
Mar 5, 2010·Biology of Reproduction·Carol B HannaXuemei Wu
Aug 1, 2006·Journal of Biomedicine & Biotechnology·Shane R HormanEline T Luning Prak
Jul 9, 2004·Cytogenetic and Genome Research·L GentileM Zuccotti
Apr 4, 2013·BMC Developmental Biology·Santanu De, Douglas Kline
Oct 27, 2010·RNA·Lucien BergeronSherif Abou Elela
Aug 24, 2010·PloS One·Lasse SinkkonenPetr Svoboda
May 5, 2006·Reproduction : the Official Journal of the Society for the Study of Fertility·Korakot NganvongpanitDawit Tesfaye
Oct 24, 2006·Journal of Biomedicine & Biotechnology·K Sue O'sheaTheresa E Gratsch
Sep 13, 2005·FEBS Letters·Alexei Aravin, Thomas Tuschl
Aug 28, 2012·Animal Reproduction Science·Radek Malik, Petr Svoboda
Jun 19, 2007·Theriogenology·K SchellanderD Tesfaye
Apr 17, 2007·Biochemical and Biophysical Research Communications·Suk Ying TsangRonald A Li
Sep 6, 2015·Theriogenology·Baofeng SuRex A Dunham
Oct 27, 2005·Molecular Reproduction and Development·Korakot NganvongpanitDawit Tesfaye
Sep 1, 2011·Reproduction in Domestic Animals = Zuchthygiene·P Bermejo-AlvarezA Gutierrez-Adan
Jan 1, 2012·Genes·Mark Baguma-NibashekaPaul R Murphy
May 16, 2014·Biology of Reproduction·Matyas FlemrPetr Svoboda
Nov 3, 2017·Frontiers in Genetics·Maxim V ZagoskinDmitry V Mukha
Jan 6, 2010·Reproduction, Fertility, and Development·Karen GoossensLuc J Peelman
Feb 12, 2010·Cold Spring Harbor Protocols·Petr Svoboda
Feb 12, 2010·Cold Spring Harbor Protocols·Petr Svoboda, Paula Stein