Long glucocorticoid-induced leucine zipper regulates human thyroid cancer cell proliferation

Cell Death & Disease
E AyroldiC Riccardi

Abstract

Long glucocorticoid-induced leucine zipper (L-GILZ) has recently been implicated in cancer cell proliferation. Here, we investigated its role in human thyroid cancer cells. L-GILZ protein was highly expressed in well-differentiated cancer cells from thyroid cancer patients and differentiated thyroid cancer cell lines, but poorly expressed in anaplastic tumors. A fusion protein containing L-GILZ, when overexpressed in an L-GILZ-deficient 8505C cell line derived from undifferentiated human thyroid cancer tissue, inhibited cellular proliferation in vitro. In addition, when this protein was injected into nude mice, in which cells from line 8505C had been transplanted, xenograft growth was reduced. Since the mitogen-activated protein kinase (MAPK) pathway is frequently hyperactivated in thyroid cancer cells as a result of the BRAFV600E or Ras mutation, we sought to further investigate the role of L-GILZ in the MAPK pathway. To this end, we analyzed L-GILZ expression and function in cells treated with MAPK inhibitors. We used 8505C cells, which have the BRAFV600E mutation, or the CAL-62 cell line, which harbors a Ras mutation. The cells were treated with the BRAF-specific drug vemurafenib (PLX4032) or the MEK1/2 inhibitor, U0126, res...Continue Reading

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Citations

Jan 22, 2020·Inflammatory Bowel Diseases·Simona RonchettiCarlo Riccardi
Feb 19, 2020·Journal of Chemotherapy·Maria Cristina MarchettiEmira Ayroldi
Jan 16, 2019·International Journal of Molecular Sciences·Xingyu XuJialiang Yang
Aug 29, 2020·The Journal of Biological Chemistry·Laurent VergnesKaren Reue

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Methods Mentioned

BETA
PCR
ChIP
immunoprecipitation
pull-down
ELISA
transfection
xenografts
xenograft
Assay
electrophoresis

Software Mentioned

Matinspector
ImageJ

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