Sep 11, 2012

Long intergenic non-coding RNA TUG1 is overexpressed in urothelial carcinoma of the bladder

Journal of Surgical Oncology
Yonghua HanZhiming Cai

Abstract

Long intergenic non-coding RNAs (lincRNAs) are a class of non-coding RNAs that regulate gene expression via chromatin reprogramming. Taurine Up-regulated Gene 1 (TUG1) is a lincRNA that is associated with chromatin-modifying complexes and plays roles in gene regulation. In this study, we determined the expression patterns of TUG1 and the cell proliferation inhibition and apoptosis induced by silencing TUG1 in urothelial carcinoma of the bladder. The expression levels of TUG1 were determined using Real-Time qPCR in a total of 44 patients with bladder urothelial carcinomas. Bladder urothelial carcinoma T24 and 5637 cells were transfected with TUG1 siRNA or negative control siRNA. Cell proliferation was evaluated using MTT assay. Apoptosis was determined using ELISA assay. TUG1 was up-regulated in bladder urothelial carcinoma compared to paired normal urothelium. High TUG1 expression levels were associated with high grade and stage carcinomas. Cell proliferation inhibition and apoptosis induction were observed in TUG1 siRNA-transfected bladder urothelial carcinoma T24 and 5637 cells. Our data suggest that lincRNA TUG1 is emerging as a novel player in the disease state of bladder urothelial carcinoma. TUG1 may have potential roles ...Continue Reading

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Mentioned in this Paper

Real-Time Polymerase Chain Reaction
Transfection
Urothelium
Apoptosis, Intrinsic Pathway
Neoplasm of Uncertain or Unknown Behavior of Bladder
RNA, Untranslated
RNA, Small Interfering
Complex (molecular entity)
Urinary Bladder Diseases
Carcinomatosis

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