Long-lasting changes in GABA responsiveness in cultured neurons

Neuroscience Letters
Joelle N ChabwineGeert Callewaert

Abstract

In neuronal cells, GABA evokes an increase in chloride conductance by activating GABA(A) and GABA(C) receptors. In mature neurons, this increase in conductance generally has a hyperpolarizing and inhibitory action. Using gramicidin-based perforated patch recordings, we show that in cultured motor neurons GABA-induced currents are significantly altered following activation of GABA receptors coinciding with changes in membrane potential. Changes in intracellular chloride concentration constituted the mechanism for this modulation. Because of low resting chloride conductance and low activity of chloride transporters, changes in intracellular chloride concentration and hence GABA response were long-lasting (time constant of recovery was about 2.5 min). Cultured dorsal horn and hippocampal neurons exhibited a similar response pattern, suggesting a general property of cultured neuronal cells. These long-lasting changes in GABA responsiveness may have major implications on neuronal excitability.

References

Nov 1, 1996·The Journal of Biological Chemistry·P SaftigB De Strooper
Sep 5, 2002·Nature Reviews. Neuroscience·Yehezkel Ben-Ari
Nov 16, 2002·The Journal of Physiology·Daniela Billups, David Attwell
Jan 28, 2003·Neuron·Allan T Gulledge, Greg J Stuart
Feb 11, 2003·Neuron·Valentin Stein, Roger A Nicoll

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Citations

Nov 2, 2012·Journal of Neurophysiology·Christopher B RansomGeorge B Richerson
Jun 4, 2010·The Journal of Neuroscience : the Official Journal of the Society for Neuroscience·Christopher B RansomGeorge B Richerson
Dec 13, 2005·Journal of Neurophysiology·Yoko Fujiwara-TsukamotoMasahiko Takada
Dec 24, 2008·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·J N ChabwineG Callewaert

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