Long-lasting coexpression of nestin and glial fibrillary acidic protein in primary cultures of astroglial cells with a major participation of nestin(+)/GFAP(-) cells in cell proliferation

Journal of Neuroscience Research
Solène Sergent-TanguyPhilippe Naveilhan

Abstract

Nestin, a currently used marker of neural stem cells, is transiently coexpressed with glial fibrillary acidic protein (GFAP) during development and is induced in reactive astrocytes following brain injury. Nestin expression has also been found in cultures of astroglial cells, but little is known about the fate and the mitotic activity of nestin-expressing cells in this in vitro model. The present study reveals a long-lasting expression of nestin in primary cultures of astroglial cells derived from the rat brain. Over 70% of the cells were nestin(+) at 12 weeks, with a large majority coexpressing the GFAP astrocytic marker. Time-course analyses supported a transition from a nestin(+)/GFAP(-) to a nestin(+)/GFAP(+) phenotype over time, which was further increased by cell cycle arrest. Interestingly, double staining with Ki67 revealed that over 90% of cycling cells were nestin(+) whereas only 28% were GFAP(+) in a population consisting of almost equivalent numbers of nestin(+) and GFAP(+) cells. These observations indicated that nestin(+)/GFAP(-) cells are actively engaged in mitotic activity, even after 2 weeks in vitro. Part of these cells might have retained properties of neural stem cells, insofar as 10% of cells in a primary ...Continue Reading

References

Oct 1, 1990·Brain Research. Developmental Brain Research·B RollandM Tardy
Jan 1, 1989·International Journal of Developmental Neuroscience : the Official Journal of the International Society for Developmental Neuroscience·A Bignami, D Dahl
Feb 1, 1988·Journal of Neurocytology·J Ciesielski-TreskaD Aunis
Apr 1, 1995·Neurobiology of Disease·R C LinO Brüstle
Jan 1, 1997·The European Journal of Neuroscience·S HolminT Mathiesen
Apr 17, 1998·Journal of Neuroscience Research·V W Wu, J P Schwartz
Jul 23, 1999·Experimental Neurology·M K CarpenterL U Wahlberg
Nov 30, 2000·Proceedings of the National Academy of Sciences of the United States of America·E D LaywellD A Steindler
Feb 28, 2001·International Journal of Developmental Neuroscience : the Official Journal of the International Society for Developmental Neuroscience·M Kálmán, B M Ajtai
May 15, 2002·International Journal of Developmental Neuroscience : the Official Journal of the International Society for Developmental Neuroscience·Rainald Schmidt-Kastner, Christian Humpel
Aug 26, 2003·Brain Research. Developmental Brain Research·Claudia RöhlJobst Sievers
Sep 3, 2003·Journal of Neuroscience Methods·Solène Sergent-TanguyPhilippe Naveilhan
Feb 3, 2005·Journal of Neurotrauma·Takehiro NakamuraToshifumi Itano

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Citations

Oct 31, 2012·Histochemistry and Cell Biology·Angela Maria FalchiMarco Piludu
Aug 3, 2013·Cellular and Molecular Neurobiology·Min-Hee YiDong Woon Kim
Jun 16, 2009·Neurotoxicity Research·Bozena Mazur-KoleckaJanusz Frackowiak
Feb 10, 2010·Journal of Cellular and Molecular Medicine·Julien RossignolLaurent Lescaudron
Jan 12, 2013·Fluids and Barriers of the CNS·Ethan S LippmannEric V Shusta
Nov 13, 2013·Journal of Molecular Neuroscience : MN·Xiangdong ChenFeng Zhang
Feb 1, 2014·Cell Death & Disease·M MorfouaceF M Vallette
Jun 28, 2011·Neurochemistry International·Jana VukovicPerry F Bartlett
Aug 21, 2007·Brain Research·Bozena Mazur-KoleckaJanusz Frackowiak
Jun 17, 2009·Journal of Neuroscience Research·Judith BuddensiekMichael Sabolek
Mar 10, 2007·Journal of Neuroscience Research·Raquel Martín-IbáñezJosep M Canals
Aug 23, 2011·Journal of Neurochemistry·Ethan S LippmannEric V Shusta
Apr 13, 2011·Biotechnology Progress·Behnam A BaghbaderaniIvar Mendez
Sep 24, 2010·Cytoskeleton·M A SilvermanG Banker
Mar 6, 2010·Journal of Neurochemistry·Till B Puschmann, Ann M Turnley
Feb 23, 2012·Progress in Retinal and Eye Research·Stefanie G WohlStefan Isenmann
May 31, 2011·Journal of Neuroscience Methods·Susan M JonesJ Paul Elliott
Apr 2, 2010·The International Journal of Neuropsychopharmacology·Stefano CabrasValeria Sogos
Jan 10, 2019·The International Journal of Neuroscience·Mariya M MikhailovaAndrey A Panteleyev
Oct 21, 2006·Neurochemical Research·Tatsuki ItohHiroyuki Ito
Aug 1, 2019·Archives of Toxicology·Karine P ReichertVera M M Morsch
Jun 9, 2021·Neurobiology of Disease·Eduard DauraTarja Joensuu

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