Long-lasting post-mortem activity of spinal microglia in situ in mice

Journal of Neuroscience Research
Payam DibajEike D Schomburg

Abstract

As CNS macrophages, microglia show a high spontaneous motility of their processes, continuously surveying their microenvironment. Upon CNS injury, microglia react by immediate cellular polarization and process extension toward the lesion site as well as by subsequent amoeboid lesion-directed migration and phagocytosis. To determine the ability of microglia to fulfill their role within distinctively lesioned tissue in the absence of life support, we investigated microglial activity and responsiveness to laser-induced axonal injuries in the spinal dorsal columns in situ after cardiac and respiratory arrest, i.e., post-mortem, in the progressively degrading nervous tissue. For this purpose, we used time-lapse two-photon laser scanning microscopy in double transgenic mice expressing enhanced green fluorescent protein in microglia and enhanced yellow fluorescent protein in projection neurons. Depending on the premortal condition of the animal, microglial activity and responsiveness remain for up to5-10 hr post-mortem. Thereby, the continuously decreasing glial reaction is independent of oxygen and glucose supply but requires residual ATP, suggesting a parasitic form of energy, such as a transmembrane uptake of ATP released from inju...Continue Reading

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Citations

Aug 2, 2012·Experimental Neurology·Helge C Johannssen, Fritjof Helmchen
Oct 13, 2010·Neuroscience Letters·Payam DibajEike D Schomburg
Apr 23, 2016·The Journal of Physiology·Tuan Leng TayMarie-Ève Tremblay
Feb 1, 2018·Proceedings of the National Academy of Sciences of the United States of America·Christian MadryDavid Attwell
Mar 1, 2019·Frontiers in Cellular Neuroscience·Hisaomi SuzukiTakahiro A Kato

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