Long-lasting production of TGF-beta1 by alveolar macrophages exposed to low doses of asbestos without apoptosis

International Journal of Immunopathology and Pharmacology
Y NishimuraH Iguchi

Abstract

Alveolar macrophages (AMs) exposed to asbestos are well known to produce TNF-alpha, which induces the production of TGF-beta1, leading to lung fibrogenesis. The present study examines the production of TGF-beta1 by AMs exposed to chrysotile B asbestos (CH) in vivo or in vitro and the relationship between TGF-beta1 production and apoptosis in cultures of AMs. Rats instilled with CH via the trachea showed increases in TNF-alpha, IL-1beta and IL-6 in the bronchoalveolar lavage fluid (BALF) 1 day after the instillation, followed by increases in TGF-beta1 and apoptotic cells 5 days after. The AMs from these BALFs produced a significantly increased amount of TGF-beta1 in culture compared to those from the control rats. The addition of 2.5 mug/cm2 of CH augmented the production of TGF-beta1 by the AMs from the control to the same level as produced by the AMs from the CH-treated rats. The apoptosis of AMs was not induced at 2.5 microg/cm2 of CH, but was drastically induced at over 12.5 microg/cm2. In contrast, the production of TGF-beta1 by AMs peaked at around 2.5 microg/cm2 of CH, and it lasted for 11 days. In addition, Bcl-2 and Bcl-xL increased in the AMs surviving under the exposure to CH. Taken together, these results indicate th...Continue Reading

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Citations

Apr 23, 2009·Neurotoxicity Research·M L CastellaniT C Theoharides
Oct 5, 2010·Annual Review of Pharmacology and Toxicology·Debra L LaskinJeffrey D Laskin
Sep 22, 2010·Toxicological Sciences : an Official Journal of the Society of Toxicology·Kelly E DuncanRobert B Devlin
Sep 21, 2010·Journal of Immunotoxicology·Megumi MaedaTakemi Otsuki
Mar 7, 2013·Environmental Health and Preventive Medicine·Yasumitsu NishimuraTakemi Otsuki
Jan 20, 2011·International Journal of Immunopathology and Pharmacology·G MazzoccoliG Vendemiale
Oct 22, 2013·Occupational and Environmental Medicine·Mari K KukkonenAri Hirvonen
Mar 23, 2021·Chemical Research in Toxicology·Glen ReidAnthony M George
Oct 26, 2021·Frontiers in Molecular Biosciences·Yicheng HeHuahao Shen

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