Long non-coding RNA AFAP1-AS1 plays an oncogenic role in promoting cell migration in non-small cell lung cancer

Cellular and Molecular Life Sciences : CMLS
Juan HeYong Peng

Abstract

Long non-coding RNA (lncRNA) plays an important role in tumor progression and metastasis. Emerging evidence indicates that lncRNA actin filament-associated protein 1-antisense RNA 1 (AFAP1-AS1) is dysregulated in certain tumors. However, the function of AFAP1-AS1 in non-small cell lung cancer (NSCLC) remains elusive. In this study, we conducted global lncRNA profiling and identified that AFAP1-AS1 is significantly upregulated in NSCLC, suggesting that AFAP1-AS1 may be important for lung cancer development. For the first time, the transcription initiation and termination sites of AFAP1-AS1 were identified by rapid amplification of cDNA ends technology, and the sequencing data indicated that AFAP1-AS1 in lung cancer cells is a novel transcript variant. Through gain- and loss-of-function studies, AFAP1-AS1 was demonstrated to promote cell migration and invasion. Mechanistically, AFAP1-AS1 functions through positively regulating the expression of AFAP1 protein. On the other hand, the expression of lncRNA AFAP1-AS1 negatively correlates with CpG methylation status of its gene promoter, identified in both lung cancer cells and patient tissues, and treatment with DNA methyltransferase inhibitor decitabine significantly activates AFAP1...Continue Reading

References

Jun 14, 2005·Lung Cancer : Journal of the International Association for the Study of Lung Cancer·Hong ChenHideki Kimura
Jun 26, 2008·Journal of Cell Science·Andrea DorfleutnerDaniel C Flynn
Jan 10, 2009·Nature Protocols·Da Wei HuangRichard A Lempicki
Feb 4, 2009·Nature Reviews. Genetics·Tim R MercerJohn S Mattick
Feb 26, 2009·Cell·Chris P PontingWolf Reik
Nov 19, 2011·Cancer Discovery·John R Prensner, Arul M Chinnaiyan
Dec 23, 2011·European Journal of Cancer : Official Journal for European Organization for Research and Treatment of Cancer (EORTC) [and] European Association for Cancer Research (EACR)·Jing WangLei Wei
Mar 1, 2012·Frontiers in Genetics·Courtney N NilandAhmad M Khalil
May 1, 2012·Trends in Genetics : TIG·Marco MagistriClaes Wahlestedt
Sep 8, 2012·Genome Research·Jennifer HarrowTim J Hubbard
Dec 4, 2013·Nature Reviews. Genetics·Alessandro Fatica, Irene Bozzoni
Jan 25, 2014·Molecular Cancer·Inga HinrichsenAngela Brieger
Feb 19, 2015·Nature Biotechnology·Mihaela PerteaSteven L Salzberg
Mar 10, 2015·Nature Methods·Daehwan KimSteven L Salzberg
Jan 1, 2015·Nature Reviews. Disease Primers·Cesare GridelliRafael Rosell
Jun 5, 2016·Biomedicine & Pharmacotherapy = Biomédecine & Pharmacothérapie·Feng WangLin Chen
Nov 5, 2016·Biomedicine & Pharmacotherapy = Biomédecine & Pharmacothérapie·Fei MaJun Ding
Nov 9, 2016·Proceedings of the National Academy of Sciences of the United States of America·Angel Diaz-LagaresManel Esteller
Jul 5, 2018·Journal of Cellular and Molecular Medicine·Zhennan YuanShenshan Jia

❮ Previous
Next ❯

Citations

May 6, 2019·Cancers·Cornelia BraicuIoana Berindan-Neagoe
Feb 29, 2020·Cancer Biotherapy & Radiopharmaceuticals·Wenwei Mao, Tingjian Li
Sep 29, 2019·Journal of Translational Medicine·Rui LiYi-Qing Qu
Apr 14, 2020·Cell Biochemistry and Function·Nanchang WuShiting Li
Jul 2, 2020·Journal of Biological Research·Chengcheng ZhaoFengchang Qiao
Aug 16, 2021·Biochimica Et Biophysica Acta. Reviews on Cancer·Ganesh Kumar BarikBhargab Kalita
Sep 1, 2021·Cancer Cell International·Biao LiuJianye Liu
Jun 17, 2019·Pathology, Research and Practice·Ming Yang, Wenbin Wei

❮ Previous
Next ❯

Methods Mentioned

BETA
RNA-seq
Assay
PCR
reverse-transcription PCR
electrophoresis

Software Mentioned

HiSat2
GENCODE
edgeR
KOBAS
R package “ Survival ”
CRAN
StringTie
TCGA
DAVID

Related Concepts

Related Feeds

Cell Migration

Cell migration is involved in a variety of physiological and pathological processes such as embryonic development, cancer metastasis, blood vessel formation and remoulding, tissue regeneration, immune surveillance and inflammation. Here is the latest research.

Cell Migration in Cancer and Metastasis

Migration of cancer cells into surrounding tissue and the vasculature is an initial step in tumor metastasis. Discover the latest research on cell migration in cancer and metastasis here.