Long non-coding RNA CASC2 improved acute lung injury by regulating miR-144-3p/AQP1 axis to reduce lung epithelial cell apoptosis

Cell & Bioscience
Hongbin LiRongqing Sun

Abstract

Apoptosis of lung epithelial cell is implicated in the pathogenesis of acute lung injury (ALI). To study the protective effect and mechanism of cancer susceptibility candidate 2 (CASC2) on reducing lung epithelial cell apoptosis after LPS inducing acute lung injury in mice. The ALI mice model was performed by intratracheally instilling with lipopolysaccharide (LPS). The CASC2 expression detected by quantitative real-time polymerase chain reaction was significantly decreased in LPS-induced A549 cell and ALI mice model. LPS induced A549 cell apoptosis, while transfection with pcDNA-CASC2 reversed the increased cell apoptosis, suggesting overexpression of CASC2 inhibited LPS-induced A549 cell apoptosis. In addition, we found that miR-144-3p expression were opposite to CASC2, while Aquaporin-1 (AQP1) expression was opposite to miR-144-3p in LPS-induced A549 cell and ALI mice model. The RNA immunoprecipitation and RNA pull-down assay demonstrated that CASC2 could function as a miR-144-3p decoy. The luciferase reporter assay revealed that AQP1 was a target of miR-144-3p in A549 cell. And then, further in vitro studied showed that CASC2 controlled AQP1 expression by regulating miR-144-3p, and LPS induced A549 cell apoptosis by regulat...Continue Reading

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Citations

Jan 7, 2020·Expert Review of Respiratory Medicine·Xianfeng ChenZhanhong Tang
Jul 7, 2018·Frontiers in Chemistry·André GomesGraça Soveral
Jul 31, 2020·International Journal of Molecular Sciences·Pál TodPéter Hamar
Jan 18, 2021·Life Sciences·Chaojin ChenZhiqiang Ye
Oct 30, 2020·Cancer Management and Research·Hui-Ling CaoZhong-Jian Chen
Mar 7, 2021·International Journal of Molecular Sciences·Inês V da Silva, Graça Soveral
Apr 14, 2021·Infection, Genetics and Evolution : Journal of Molecular Epidemiology and Evolutionary Genetics in Infectious Diseases·Yan Jiang, Wei Zhang

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Methods Mentioned

BETA
transfection
Assay
PCR
immunoprecipitation
flow cytometry
flow
pull-down
pharmacotherapy

Software Mentioned

SPSS

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