Long non-coding RNA DSCR8 acts as a molecular sponge for miR-485-5p to activate Wnt/β-catenin signal pathway in hepatocellular carcinoma.

Cell Death & Disease
Yufeng WangQingguang Liu

Abstract

Previous evidences reveal that long non-coding RNA (lncRNA) down syndrome critical region 8 (DSCR8) involves in the progression of multiple cancers. However, the exact expression, function, and mechanism of DSCR8 in hepatocellular carcinoma (HCC) remain uncovered. In this study, real-time PCR in HCC tissues and cell lines indicated that DSCR8 expression was upregulated, while miR-485-5p was downregulated. MTT assay, plate clone formation, Edu assay, flow cytometry, and in vivo experiments indicated that DSCR8 promoted HCC cell proliferation and cycle, whereas accelerated cell apoptosis. Luciferase reporter gene assay, RIP assay, and rescue experiments demonstrated that DSCR8 functioned as a competing endogenous RNA (ceRNA) by sponging miR-485-5p in HCC cells. Furthermore, gain- and loss-of-function studies showed that miR-485-5p activated Wnt/β-catenin signal pathway by targeting Frizzled-7 (FZD7). Moreover, DSCR8 activated Wnt/β-catenin signal pathway to promote HCC progression by DSCR8/miR-485-5p/FZD7 axis. Statistical analysis revealed that DSCR8 and miR-485-5p were closely related to some malignant clinicopathological features and 5-year survival rates of HCC patients. Taken together, the present study reports for the first...Continue Reading

References

Mar 29, 2002·International Journal of Cancer. Journal International Du Cancer·Nicole J W de WitGoos N P van Muijen
Mar 17, 2007·Clinical Cancer Research : an Official Journal of the American Association for Cancer Research·John Ian RisingerJ Carl Barrett
Jun 10, 2015·Biomedicine & Pharmacotherapy = Biomédecine & Pharmacothérapie·Xiangjun SunYutong Wang
Mar 24, 2016·Tumour Biology : the Journal of the International Society for Oncodevelopmental Biology and Medicine·Yufeng WangCheng Guo
Apr 2, 2017·Biomedicine & Pharmacotherapy = Biomédecine & Pharmacothérapie·Jiawen WuDingwei Zhang
Apr 20, 2017·Journal of Cell Science·Hanna BergerAnnette Borchers
Apr 21, 2017·Frontiers in Cell and Developmental Biology·Hanna BergerAnnette Borchers
Apr 26, 2017·Journal of Hepatology·Marcel KlingenbergTushar Patel
Apr 27, 2017·Molecular Cancer Research : MCR·Shanta M MesserliRatan D Bhardwaj
Jan 9, 2018·Lancet·Alejandro FornerJordi Bruix
Jan 10, 2018·Hepatobiliary Surgery and Nutrition·Di-Yang XieQiang Gao
Jan 11, 2018·Nature Reviews. Gastroenterology & Hepatology·Chun-Ming WongIrene Oi-Lin Ng
Jan 18, 2018·Arquivos Brasileiros De Cirurgia Digestiva : ABCD = Brazilian Archives of Digestive Surgery·Marcio F ChedidAljamir D Chedid
Feb 15, 2018·Endocrine-related Cancer·Carolyn M Klinge
Mar 8, 2018·Journal of Experimental & Clinical Cancer Research : CR·Zhikui LiuKangsheng Tu
Apr 28, 2018·International Journal of Molecular Sciences·Jia Jia Chan, Yvonne Tay

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Citations

Jul 25, 2019·Journal of Clinical Medicine·Saumik BiswasSubrata Chakrabarti
Aug 28, 2020·World Journal of Gastroenterology : WJG·Zhao-Shan NiuLi-Mei-Li Tian
Apr 17, 2020·Molecular Cancer·Zhao HuangCanhua Huang
Dec 19, 2020·Journal of Cellular and Molecular Medicine·Mingjun YuShijia Yu
Oct 12, 2019·Clinica Chimica Acta; International Journal of Clinical Chemistry·Dandan LiJinming Li
Oct 17, 2020·Cancer Cell International·Gang XuZhong-Tao Zhang
Feb 19, 2021·Journal of Experimental & Clinical Cancer Research : CR·Liang WangQingguang Liu
Dec 16, 2018·Biomedicine & Pharmacotherapy = Biomédecine & Pharmacothérapie·Lei ZhangKangsheng Tu
Sep 22, 2020·Biochemistry and Cell Biology = Biochimie Et Biologie Cellulaire·Fen-Rong ChenLei Dong
Jun 21, 2020·Biomedicine & Pharmacotherapy = Biomédecine & Pharmacothérapie·Qi YouHaitao Yuan
Mar 26, 2019·Biomedicine & Pharmacotherapy = Biomédecine & Pharmacothérapie·Xueli WangLei Wang

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Methods Mentioned

BETA
PCR
flow cytometry
immunoprecipitation
RIP
Assay

Software Mentioned

TargetScan
miRDB
Graphpad Prism
SPSS

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