Long non-coding RNA EPIC1 promotes human lung cancer cell growth

Biochemical and Biophysical Research Communications
Bing ZhangYu-Xin Lv

Abstract

Long non-coding RNA (LncRNA) EPIC1 (Lnc-EPIC1) is a MYC-interacting LncRNA. In the present study, the expression and potential function of Lnc-EPIC1 in human lung cancer cells are tested. We show that Lnc-EPIC1 expression is significantly higher in established/primary human lung cancer cells than that in human lung epithelial cells. Lnc-EPIC1 is also elevated in human lung cancer tissues. Silencing of Lnc-EPIC1 by targeted siRNA significantly inhibited human lung cancer cell growth, survival and proliferation, whiling inducing G1-S cell cycle arrest and cell apoptosis. MYC targets, including Cyclin A1, CDC20 and CDC45, were downregulated by Lnc-EPIC1 siRNA. MYC knockout by CRISPR/Cas-9 gene-editing method mimicked actions by Lnc-EPIC1 siRNA in A549 cells. Conversely, forced overexpression of Lnc-EPIC1 by a lentiviral construct increased expression of MYC targets to promote A549 cell growth. Lnc-EPIC1 directly associated with MYC protein in the nuclei of A549 cells. Significantly MYC knockout abolished Lnc-EPIC1 silencing- or overexpression-induced actions in human lung cancer cells. Together, our results show that Lnc-EPIC1 promotes human lung cancer cell growth possibly by targeting MYC.

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Citations

Dec 4, 2019·Aging·Li-Na ZhouFang Liu
Mar 4, 2020·Journal of Cancer Research and Clinical Oncology·Sixuan LiZhihua Yin
Aug 15, 2019·Cell Death & Disease·Xuebang ZhangYuxia Duan
Aug 13, 2020·Therapeutic Advances in Medical Oncology·Yaqian XuJinsong Lu
Apr 26, 2020·Journal of Clinical Medicine·Dino BekricChristian Mayr
Mar 29, 2020·Technology in Cancer Research & Treatment·Jing-Jing ZhengHua Shi
Apr 4, 2021·Cancers·Leila JahangiriSuzanne D Turner
Apr 8, 2021·Cell Death & Disease·Ying-Chen XiaBen-Tong Yu
Apr 17, 2021·Essays in Biochemistry·Huiwen Yan, Pengcheng Bu
Jun 29, 2021·Frontiers in Oncology·Jian-Hua ZhaGuo-Qiu Xu
Mar 25, 2019·Biochemical and Biophysical Research Communications·Hang LiSaijun Fan
Aug 28, 2021·Biomedicines·Ekaterina Mikhailovna StasevichAnton Markovich Schwartz
Dec 8, 2019·Biochemical and Biophysical Research Communications·Peng XiaHongwei Zhang
Jan 21, 2022·The Kaohsiung Journal of Medical Sciences·Hao HuChang-Ying Guo

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