Long non-coding RNA GHET1 promotes human breast cancer cell proliferation, invasion and migration via affecting epithelial mesenchymal transition

Cancer Biomarkers : Section a of Disease Markers
Rui SongTao Hai

Abstract

Breast cancer is a common malignancy in women and long non-coding RNAs (lncRNAs) have been shown to play key roles in the development and progression of breast cancer. In the present study, we examined the biological role of lncRNA gastric carcinoma highly expressed transcript 1 (GHET1) in breast cancer. The expression of GHET1 was determined by qRT-PCR assay; CCK-8, colony formation, Transwell invasion and migration assays detected breast cancer cell proliferation, invasion and migration; cell apoptosis and cell cycle were determined by flow cytometry; protein levels were determined by western blot assay. GHET1 was up-regulated in breast cancer tissues and cell lines, and the up-regulation of GHET1 was positively correlated with larger tumor size, advanced clinical stage, lymph node metastasis and shorter overall survival. Knockdown of GHET1 suppressed cell proliferation, invasion and migration, and induced apoptosis and G0/G1 cell cycle arrest in MCF-cells. Knockdown of GHET1 also suppressed the protein levels of N-cadherin, vimentin, and decreased the protein level of E-cadherin in MCF-7 cells. On the other hand, overexpression of GHET1 promoted cell proliferation, invasion and migration, and inhibited cell apoptosis and inc...Continue Reading

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Citations

Apr 16, 2019·Technology in Cancer Research & Treatment·Tianzhu ZhangZhongxin Lu
May 24, 2019·Journal of Cellular and Molecular Medicine·Adheesh BhandariDuping Huang
Nov 27, 2018·Cancer Biomarkers : Section a of Disease Markers·Wei YangZhandong Zhang
Apr 14, 2020·Cancer Cell International·Dingding WangHonggang Liu
Oct 20, 2018·Expert Review of Molecular Diagnostics·Konstantina PanoutsopoulouAndreas Scorilas
Nov 22, 2018·Cancer Cell International·Xu BinTang Binbin
Feb 26, 2021·Pathology, Research and Practice·Soudeh Ghafouri-FardMohammad Taheri
Jul 29, 2020·Current Pharmaceutical Biotechnology·Arash PoursheikhaniAmirhossein Sahebkar

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