Long non-coding RNA H19 promotes colorectal cancer metastasis via binding to hnRNPA2B1.

Journal of Experimental & Clinical Cancer Research : CR
Yuhui ZhangChanghua Zhang

Abstract

Long non-coding RNA H19 was demonstrated to be significantly correlated with tumor metastasis. However, the specific functions of H19 in colorectal cancer (CRC) metastasis and the underlying mechanism are still largely unclear. Use public database to screen the potential lncRNA crucial for metastasis in colorectal cancer. The expression of H19 in clinical CRC specimens was detected by qRT-PCR. The effect of H19 on the metastasis of CRC cells was investigated by transwell, wound healing assays, CCK-8 assays and animal studies. The potential proteins binding to H19 were identified by LC-MS and verified by RNA immunoprecipitation (RIP). The expression of indicated RNA and proteins were measured by qRT-PCR or western blot. We found the expression of lncRNA H19 was significantly upregulated in primary tumor and metastatic tissues, correlated with poor prognosis in CRC. Ectopic H19 expression promoted the metastasis of colorectal cancer cells in vitro and in vivo, and induced epithelial-to-mesenchymal transition (EMT). Mechanistically, H19 directly bound to hnRNPA2B1. Knockdown of hnRNPA2B1 attenuated the H19-induce migration and invasion in CRC cells. Furthermore, H19 stabilized and upregulated the expression of Raf-1 by facilitated...Continue Reading

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Citations

Dec 4, 2020·Journal of Experimental & Clinical Cancer Research : CR·Xue-Ting HuXiang Xu
Aug 2, 2020·Seminars in Cancer Biology·Ammad Ahmad FarooqiMassimo Mallardo
Mar 7, 2021·Non-coding RNA·Didem Karakas, Bulent Ozpolat
Mar 25, 2021·Journal of Experimental & Clinical Cancer Research : CR·Yuhui ZhangChanghua Zhang
Jul 20, 2021·Frontiers in Cell and Developmental Biology·Kexin LiWei Cui

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Datasets Mentioned

BETA
GSE50760

Methods Mentioned

BETA
transfection
PCR
electrophoresis
pull-down
Pull Down
immunoprecipitation
RNA-seq
RIP

Software Mentioned

SPSS
Gene Set Enrichment Analysis ( GSEA
GSEA

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