Long non-coding RNA H19/SAHH axis epigenetically regulates odontogenic differentiation of human dental pulp stem cells

Cellular Signalling
Li ZengYixiang Wang

Abstract

Long noncoding RNAs (lncRNAs) are emerging as important regulators in molecular processes and may play vital roles in odontogenic differentiation of human dental pulp stem cells (hDPSCs). However, their functions remain to be elucidated. As lncRNA H19 is one of the most classical lncRNA, which plays essential roles in cellular differentiation, thus we explored the effects and mechanisms of H19 in odontogenic differentiation of hDPSCs. Stable overexpression and knockdown of H19 in hDPSCs were constructed using recombinant lentiviruses containing H19 and short hairpin-H19 expression cassettes, respectively. Alkaline phosphatase (ALP) assay, Alizarin red staining assay, von kossa staining, quantitative polymerase chain reaction (qPCR), Western blot analysis, and immunofluorescent staining results indicated that overexpression of H19 in hDPSCs positively regulates the odontogenic differentiation of hDPSCs, while knockdown of H19 in hDPSCs inhibits odontogenic differentiation of hDPSCs. Further, we found that H19 promotes the odontogenic differentiation of hDPSCs through S-adenosylhomocysteine hydrolase (SAHH) epigenetically regulates the methylation and expression of distal-less homeobox (DLX3) gene. Herein, for the first time, we ...Continue Reading

Citations

Nov 6, 2020·Stem Cells International·Dan ZhouWei Du
May 29, 2021·Stem Cells International·Jingqiu ChenJun Zhang
Jun 18, 2021·World Journal of Stem Cells·Jing-Yao YinDan-Dan Ma
Jul 10, 2021·Frontiers in Cell and Developmental Biology·Bo LiYi Men
Jul 15, 2021·Seminars in Cell & Developmental Biology·Johnah Galicia, Asma A Khan

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