Long Non-Coding RNA HAND2-AS1 Acts as a Tumor Suppressor in High-Grade Serous Ovarian Carcinoma.

International Journal of Molecular Sciences
Priyanka GokulnathMariastella Zannini

Abstract

Long non-coding RNAs (lncRNAs) are increasingly being identified as crucial regulators in pathologies like cancer. High-grade serous ovarian carcinoma (HGSC) is the most common subtype of ovarian cancer (OC), one of the most lethal gynecological malignancies. LncRNAs, especially in cancers such as HGSC, could play a valuable role in diagnosis and even therapy. From RNA-sequencing analysis performed between an OC cell line, SKOV3, and a Fallopian Tube (FT) cell line, FT194, an important long non-coding RNA, HAND2 Anti sense RNA 1 (HAND2-AS1), was observed to be significantly downregulated in OCs when compared to FT. Its downregulation in HGSC was validated in different datasets and in a panel of HGSC cell lines. Furthermore, this study shows that the downregulation of HAND2-AS1 is caused by promoter hypermethylation in HGSC and behaves as a tumor suppressor in HGSC cell lines. Since therapeutic relevance is of key importance in HGSC research, for the first time, HAND2-AS1 upregulation was demonstrated to be one of the mechanisms through which HDAC inhibitor Panobinostat could be used in a strategy to increase HGSC cells' sensitivity to chemotherapeutic agents currently used in clinical trials. To unravel the mechanism by which H...Continue Reading

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Citations

Nov 27, 2020·International Journal of Molecular Sciences·Eleonora A BragaNikolay E Kushlinskii
Jan 21, 2021·Journal of Orthopaedic Surgery and Research·Zhenxing SiJinglong Yan
Feb 9, 2021·Biomedicine & Pharmacotherapy = Biomédecine & Pharmacothérapie·Xinyu GuHaihong Zhu

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Datasets Mentioned

BETA
GSE69428

Methods Mentioned

BETA
RNA-seq
transfection
histone acetylation
RAP
PCR
Assay
RNAseq

Software Mentioned

ClueGO
GDCRNATools R package
Biorender
GraphPad Prism
Gene Expression Profiling Interactive Analysis ( GEPIA )
GDCRNATools
jVenn
cBioportal
DiseaseMeth
Cytoscape

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