Long non-coding RNA HIF1A-AS1 is upregulated in intracranial aneurysms and participates in the regulation of proliferation of vascular smooth muscle cells by upregulating TGF-β1

Experimental and Therapeutic Medicine
Jiaming XuJingjing Gu

Abstract

Long non-coding (lnc)RNA hypoxia inducible factor 1α-antisense RNA 1 (HIF1A-AS1) not only participates in different types of malignancies, but also serves pivotal roles in thoracic aortic aneurysms, which suggests its possible involvement in intracranial aneurysms. Therefore, the present study aimed to investigate its involvement in intracranial aneurysms. Expression levels of HIF1A-AS1 and transforming growth factor (TGF)-β1 in the blood of patients with intracranial aneurysms and healthy controls were detected using reverse transcription-quantitative polymerase chain reaction. The diagnostic value of blood HIF1A-AS1 for intracranial aneurysms was analyzed using receiver operating characteristic curve analysis. A HIF1A-AS1 expression vector was constructed and transfected into human vascular smooth muscle cells (VSMCs) and the effects on cell proliferation and TGF-β1 expression were explored using the Cell Counting kit-8 assay and western blot analysis, respectively. Upregulated HIF1A-AS1 expression levels in blood were observed in patients with intracranial aneurysms when compared with controls. Notably, upregulated HIF1A-AS1 expression effectively distinguished patients with intracranial aneurysms from healthy controls. Furt...Continue Reading

References

Feb 16, 2002·Methods : a Companion to Methods in Enzymology·K J Livak, T D Schmittgen
Jul 18, 2003·Lancet·David O WiebersUNKNOWN International Study of Unruptured Intracranial Aneurysms Investigators
Jun 16, 2009·American Journal of Physiology. Heart and Circulatory Physiology·Shirling TsaiK Craig Kent
Dec 15, 2010·Gynecologic and Obstetric Investigation·Paola De SanctisCinzia Zucchini
Jun 25, 2013·Cancer Letters·Xuefei ShiYong Song
Dec 11, 2013·Translational Stroke Research·Robert M StarkeAaron S Dumont
May 3, 2014·Breast Cancer Research and Treatment·Mark R Pickard, Gwyn T Williams
May 31, 2014·European Journal of Cardio-thoracic Surgery : Official Journal of the European Association for Cardio-thoracic Surgery·Shuwei WangZhiyun Xu
Apr 12, 2015·Journal of Neurointerventional Surgery·Jeff R AndersonChristof Karmonik
Apr 18, 2015·Frontiers in Genetics·Aparna DuggiralaCostanza Emanueli
Aug 1, 2015·AJNR. American Journal of Neuroradiology·J R CebralA M Robertson
Dec 17, 2015·Nature Reviews. Neurology·Miikka Korja, Jaakko Kaprio
Jun 9, 2017·Scientific Reports·Xiaolong TuJunfeng Zhang

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Citations

Jan 31, 2020·Oxidative Medicine and Cellular Longevity·Qidong CaoChunli Song

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Methods Mentioned

BETA
imaging techniques
PCR
transfection

Software Mentioned

GraphPad Prism
ImageJ

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