Long non-coding RNA HOTAIR knockdown enhances radiosensitivity through regulating microRNA-93/ATG12 axis in colorectal cancer.

Cell Death & Disease
Yingqiang LiuHong Ge

Abstract

Colorectal cancer (CRC) is a global healthcare problem. Radioresistance is a huge setback for CRC radiotherapy. In this text, the roles and molecular mechanisms of long non-coding RNA HOTAIR in CRC tumorigenesis and radioresistance were further investigated. ATG12 mRNA, HOTAIR, and microRNA-93 (miR-93) levels were measured by quantitative reverse transcription polymerase chain reaction (RT-qPCR) assay. Protein levels of LC3 I, LC3 II, p62, ATG12, cleaved caspase 3, Bax, and Bcl-2 were detected by western blotting assay in cells and were examined by immunohistochemistry (IHC) assay in tissues. Cell survival fractions, viability, and apoptotic rates were determined by clonogenic survival assay, CCK-8 assay, and flow cytometry analysis, respectively. The relationships of HOTAIR, miR-93, and ATG12 were tested by bioinformatics analysis and luciferase reporter assay. Mouse xenograft tumor models were established to investigate the influence of HOTAIR knockdown on CRC radioresistance in vivo. We found that HOTAIR expression was markedly upregulated in plasma from CRC patients after radiotherapy and CRC cells after irradiation. HOTAIR knockdown, miR-93 overexpression, or ATG12 silencing weakened cell viability, induced cell apoptosis,...Continue Reading

References

Nov 19, 2011·Cancer Discovery·John R Prensner, Arul M Chinnaiyan
Nov 15, 2013·Lancet·Hermann BrennerChristian Peter Pox
Jan 17, 2014·Nature·Yvonne TayPier Paolo Pandolfi
May 23, 2014·Current Pharmaceutical Biotechnology·Lutfi TutarYusuf Tutar
May 31, 2014·World Journal of Gastroenterology : WJG·Weiqi TanTheresa M C Tan
Jul 17, 2014·Neoplasma·B CaiS Zhang
Nov 6, 2014·Tumour Biology : the Journal of the International Society for Oncodevelopmental Biology and Medicine·Qingchao TangXishan Wang
Dec 31, 2014·Tumour Biology : the Journal of the International Society for Oncodevelopmental Biology and Medicine·Li JingQiu Haifeng
Aug 27, 2015·International Journal of Molecular Sciences·Jun WangZhen-Ning Wang
Sep 12, 2015·Journal of Medical Genetics·Xiaolong QiWang Ma
Dec 15, 2015·Journal of Molecular and Cellular Cardiology·Shashi Kumar Gupta, Thomas Thum
Jan 1, 2015·Nature Reviews. Disease Primers·Ernst J KuipersToshiaki Watanabe
Aug 31, 2016·Advances in Experimental Medicine and Biology·Daniele FanaleAntonio Russo
May 14, 2017·Experimental Cell Research·Yuan XiangTong-Cun Zhang
May 26, 2017·Methods in Molecular Biology·Min XueBin Shan
Feb 23, 2018·International Journal of Molecular Sciences·Elżbieta PawłowskaJanusz Blasiak
Dec 21, 2018·Journal of Cellular Physiology·Hossein AbdeahadSeyed Mahdi Hassanian

❮ Previous
Next ❯

Citations

Feb 6, 2021·Cancer Management and Research·Lin ChenQi Jiang
Nov 17, 2020·Frontiers in Oncology·Jose G Alvarez-MeythalerMatilde E LLeonart
Jan 11, 2021·Pathology, Research and Practice·Kaveh EbahimzadehSoudeh Ghafouri-Fard
Mar 29, 2021·Experimental and Molecular Pathology·Min GuManling Tang
Apr 23, 2021·Biochemical and Biophysical Research Communications·Hai-Rong ZhangZhong-Xue Fu
Apr 27, 2021·Pharmacology & Therapeutics·Li ChenHaibo Xu
May 20, 2021·Cancer Management and Research·Li HeXiaobing Li
Jul 3, 2021·Cancers·Paulína Pidíková, Iveta Herichová
Aug 10, 2021·Frontiers in Oncology·Xiaoru XinTiejun Zhao

❮ Previous
Next ❯

Methods Mentioned

BETA
xenograft
PCR
Protein Assay
electrophoresis
flow cytometry
transfection

Software Mentioned

Quantity One

Related Concepts

Related Feeds

Apoptosis

Apoptosis is a specific process that leads to programmed cell death through the activation of an evolutionary conserved intracellular pathway leading to pathognomic cellular changes distinct from cellular necrosis

Autophagy & Model Organisms

Autophagy is a cellular process that allows degradation by the lysosome of cytoplasmic components such as proteins or organelles. Here is the latest research on autophagy & model organisms