Long Non-Coding RNA HOXA-AS2 Regulates Malignant Glioma Behaviors and Vasculogenic Mimicry Formation via the MiR-373/EGFR Axis

Cellular Physiology and Biochemistry : International Journal of Experimental Cellular Physiology, Biochemistry, and Pharmacology
Yana GaoYixue Xue

Abstract

Vasculogenic mimicry (VM) has been reported to be a novel glioma neovascularization process. Anti-VM therapy provides new insight into glioma clinical management. In this study, we revealed the role of the long non-coding RNA HOXA cluster antisense RNA 2 (HOXA-AS2) in malignant glioma behaviors and VM formation. Quantitative real-time PCR was performed to determine the expression levels of HOXA-AS2 in glioma samples and glioblastoma cell lines. CD34-periodic acid-Schiff dual-staining was performed to assess VM in glioma samples. CCK-8, transwell, and Matrigel tube formation assays were performed to measure the effects of HOXA-AS2 knockdown on cell viability, migration, invasion, and VM tube formation, respectively. RNA immunoprecipitation, dual-luciferase reporter and Western blot assays were performed to explore the molecular mechanisms underlying the functions of HOXS-AS2 in glioblastoma cells. A nude mouse xenograft model was used to investigate the role of HOXA-AS2 in xenograft glioma growth and VM density. Student's t-tests, one-way ANOVAs followed by Bonferroni posthoc tests, and chi-square tests were used for the statistical analyses. HOXA-AS2 was upregulated in glioma samples and cell lines and was positively correlated...Continue Reading

Citations

Aug 23, 2018·Journal of Cellular Physiology·Gerardo BottiMonica Cantile
Apr 18, 2019·Journal of Cellular Biochemistry·Jie WuYijian Zhang
Mar 3, 2020·Molecular Oncology·Jia HanChen Huang
Oct 1, 2020·Journal of Experimental & Clinical Cancer Research : CR·Xianjie JiangWei Xiong
May 10, 2019·Journal of Cellular Biochemistry·Seyed Ali MirhosseiniJafar Amani
Jan 30, 2020·Frontiers in Oncology·Olga N Hernández de la CruzÁngeles Carlos-Reyes
Mar 15, 2020·Journal of Hematology & Oncology·Qingxi LuoChaojun Duan
Oct 16, 2019·Frontiers in Oncology·Lízbeth Ayala-DomínguezMarcela Lizano
Dec 20, 2020·Biomedicine & Pharmacotherapy = Biomédecine & Pharmacothérapie·Bin XuJunfei Shao
Dec 31, 2020·Cancers·Ana PaçoRenata Freitas
Jul 22, 2021·Brazilian Journal of Medical and Biological Research = Revista Brasileira De Pesquisas Médicas E Biológicas·Wenli ChenChuangxin Liao
Sep 14, 2021·Artificial Cells, Nanomedicine, and Biotechnology·Hong-Jun XieTre Sang

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