Long non-coding RNA LINC00968 attenuates drug resistance of breast cancer cells through inhibiting the Wnt2/β-catenin signaling pathway by regulating WNT2.

Journal of Experimental & Clinical Cancer Research : CR
Dian-Hui XiuXue-Feng Li

Abstract

Breast cancer is one the most common cancers, making it the second leading cause of cancer-related death among women. Long non-coding RNAs (lncRNAs), with tightly regulated expression patterns, also serve as tumor suppressor during tumorigenesis. The present study aimed to elucidate the role of LINC00968 in breast cancer via WNT2-mediated Wnt2/β-catenin signaling pathway. Breast cancer chip GSE26910 was utilized to identify differential expression in LINC00968 and WNT2. The possible relationship among LINC00968, transcriptional repressor HEY and WNT2 was analyzed and then verified. Effects of LINC00968 on activation of the Wnt2/β-catenin signaling pathway was also tested. Drug resistance, colony formation, cell migration, invasion ability and cell apoptosis after transfection were also determined. Furthermore, tumor xenograft in nude mice was performed to test tumor growth and weight in vivo. WNT2 expression exhibited at a high level, whereas LINC00968 at a low expression in breast cancer which was also associated with poor prognosis in patients. LINC00968 targeted and negatively regulated WNT2 potentially via HEY1. Either overexpressed LINC00968 or silenced inhibited activation of the Wnt2/β-catenin signaling pathway, thereby ...Continue Reading

References

May 2, 2006·Statistical Applications in Genetics and Molecular Biology·Gordon K Smyth
Oct 25, 2006·BMC Bioinformatics·André FujitaMari Cleide Sogayar
Sep 6, 2007·Journal of Clinical Oncology : Official Journal of the American Society of Clinical Oncology·Kerry S CourneyaDonald C McKenzie
Dec 7, 2007·Cancer Biology & Therapy·Maciej WieczorekMonika Lamparska-Przybysz
Sep 20, 2011·Biochemical and Biophysical Research Communications·Leanne C CooperGregory L Blatch
Feb 9, 2013·Nucleic Acid Therapeutics·Marc S Weinberg, Kevin V Morris
May 25, 2013·Nucleic Acids Research·Jing WangBing Zhang
Sep 26, 2014·Molecular Cancer Research : MCR·Gabriel EadesQun Zhou
Oct 20, 2014·Tumour Biology : the Journal of the International Society for Oncodevelopmental Biology and Medicine·Hyun Ae JungYoung-Hyuck Im
Jun 28, 2015·Molecular and Cellular Endocrinology·Hui ZhangChangzhong Li
Oct 30, 2015·CA: a Cancer Journal for Clinicians·Carol E DeSantisAhmedin Jemal
Dec 8, 2015·CA: a Cancer Journal for Clinicians·Carolyn D RunowiczPatricia A Ganz
Dec 15, 2015·Tumour Biology : the Journal of the International Society for Oncodevelopmental Biology and Medicine·Sara MalihNarges Malih
Feb 18, 2016·Tumour Biology : the Journal of the International Society for Oncodevelopmental Biology and Medicine·Yao-Min ChenPeifen Fu
May 21, 2016·Arteriosclerosis, Thrombosis, and Vascular Biology·Helen WilliamsSarah J George
Jul 26, 2017·Molecular Cancer·Shouping XuDa Pang
Sep 20, 2017·Journal of Cellular Physiology·Yíng WangHai-Bo Hu
Jan 31, 2018·Molecular Cancer Research : MCR·Kirsten M TracyJane B Lian
May 20, 2018·Journal of Hematology & Oncology·Ingrid SpaanVictor Peperzak

❮ Previous
Next ❯

Citations

Mar 20, 2020·Molecular Cancer·Francesca CrudeleStefano Volinia
Jul 20, 2019·Journal of Experimental & Clinical Cancer Research : CR·Ling DengDehua Wu
Aug 9, 2020·OncoTargets and Therapy·Tonghua DuBin Liu
Jan 11, 2021·Pathology, Research and Practice·Kaveh EbahimzadehSoudeh Ghafouri-Fard
Feb 26, 2021·Pathology, Research and Practice·Soudeh Ghafouri-FardMohammad Taheri
Apr 4, 2021·Biomolecules·Nadia Flores-HuertaCésar López-Camarillo
Sep 27, 2020·Pathology, Research and Practice·Hui ZhangYanli Li
Jul 10, 2021·Digestive and Liver Disease : Official Journal of the Italian Society of Gastroenterology and the Italian Association for the Study of the Liver·Zhenzhen GaoYanhong Gu
Aug 31, 2021·Signal Transduction and Targeted Therapy·Fanyuan YuLing Ye

❮ Previous
Next ❯

Datasets Mentioned

BETA
GSE26910

Methods Mentioned

BETA
chip
surgical resection
PCR
transfection
electrophoresis
immunoprecipitation
RIP
Assay
xenograft
ELISA

Software Mentioned

R
MEM
Affy package
LncMAP
SPSS
Limma

Related Concepts

Related Feeds

Cell Migration in Cancer and Metastasis

Migration of cancer cells into surrounding tissue and the vasculature is an initial step in tumor metastasis. Discover the latest research on cell migration in cancer and metastasis here.

Apoptosis

Apoptosis is a specific process that leads to programmed cell death through the activation of an evolutionary conserved intracellular pathway leading to pathognomic cellular changes distinct from cellular necrosis

Adherens Junctions

An adherens junction is defined as a cell junction whose cytoplasmic face is linked to the actin cytoskeleton. They can appear as bands encircling the cell (zonula adherens) or as spots of attachment to the extracellular matrix (adhesion plaques). Adherens junctions uniquely disassemble in uterine epithelial cells to allow the blastocyst to penetrate between epithelial cells. Discover the latest research on adherens junctions here.

Cell Migration

Cell migration is involved in a variety of physiological and pathological processes such as embryonic development, cancer metastasis, blood vessel formation and remoulding, tissue regeneration, immune surveillance and inflammation. Here is the latest research.

Apoptosis in Cancer

Apoptosis is an important mechanism in cancer. By evading apoptosis, tumors can continue to grow without regulation and metastasize systemically. Many therapies are evaluating the use of pro-apoptotic activation to eliminate cancer growth. Here is the latest research on apoptosis in cancer.

Cadherins and Catenins

Cadherins (named for "calcium-dependent adhesion") are a type of cell adhesion molecule (CAM) that is important in the formation of adherens junctions to bind cells with each other. Catenins are a family of proteins found in complexes with cadherin cell adhesion molecules of animal cells: alpha-catenin can bind to β-catenin and can also bind actin. β-catenin binds the cytoplasmic domain of some cadherins. Discover the latest research on cadherins and catenins here.

Breast Tumorigenesis

Breast tumorigenesis involves the production or formation of tumor(s) in breast tissue. Discover the latest research on breast tumorigenesis here.

Breast Cancer: Chemo-Resistance

Some cancers are difficult to treat and aggressive including the "triple-negative" breast cancer. This type of cancer is chemoresistant even before chemotherapy begins. Here are the latest discoveries chemo-resistance in breast cancer.