Long non-coding RNA LINC01426 facilitates glioblastoma progression via sponging miR-345-3p and upregulation of VAMP8

Cancer Cell International
Jingwei CaoZhiqiang Su

Abstract

Long non-coding RNAs (lncRNAs) has been extensively reported play important roles in regulating the development and progression of cancers, including Glioblastoma (GBM). LINC01426 is a novel lncRNA that has been identified as an oncogenic gene in GBM. Herein, we attempted to elucidate the detailed functions and underlying mechanisms of LINC01426 in GBM. LINC01426 expression in GBM cell lines and tissues were detected by quantitative real-time PCR (qRT-PCR). Cell Counting Kit-8 (CCK8) assays, colony formation assays, subcutaneous tumor formation assays were utilized to investigate the biological functions of LINC01426 in GBM. Dual-luciferase reporter assays, RNA immunoprecipitation (RIP) and bioinformatic analysis were performed to determine the underlying mechanisms. LINC01426 is up-regulated in malignant GBM tissues and cell lines and it is capable to promote GBM cell proliferation and growth. Mechanistically, LINC01426 serves as a molecular sponge to sequester the miR345-3p and thus enhancing the level of VAMP8, an oncogenic coding gene, to promote GBM progression. Our results revealed the detailed mechanisms of LINC01426 facilitated cell proliferation and growth in GBM and report the clinical value of LINC01426 for GBM progn...Continue Reading

References

May 22, 2013·Medical Hypotheses·Yuanzhao SunDai Zhou
Jul 6, 2013·Cell·Igor Ulitsky, David P Bartel
Mar 11, 2015·Cancer Letters·Dong HanXu Gao
Mar 31, 2015·Cancers·Meena Jhanwar-UniyalRaj Murali
Mar 16, 2016·International Journal of Molecular Sciences·Ahmed S BayoumiIl-Man Kim
May 29, 2016·Molecular Cancer·Nenad BartonicekMarcel E Dinger
Jul 14, 2017·Cancer Research·Arunoday BhanSubhrangsu S Mandal

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Methods Mentioned

BETA
surgical resections
transfection
PCR
immunoprecipitation
RIP

Software Mentioned

GEPIA
Targetscan
Targetscan bank

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