Long Non-Coding RNA LUCAT1 Promotes Proliferation and Invasion in Clear Cell Renal Cell Carcinoma Through AKT/GSK-3β Signaling Pathway

Cellular Physiology and Biochemistry : International Journal of Experimental Cellular Physiology, Biochemistry, and Pharmacology
Zaosong ZhengWenlian Xie

Abstract

Long non-coding RNAs (lncRNAs) have emerged as new regulators and biomarkers in several cancers. However, few lncRNAs have been well characterized in clear cell renal cell carcinoma (ccRCC). We investigated the lncRNA expression profile by microarray analysis in 5 corresponding ccRCC tissues and adjacent normal tissues. Lung cancer-associated transcript 1 (LUCAT1) expression was examined in 90 paired ccRCC tissues by real-time PCR and validated by The Cancer Genome Atlas (TCGA) database. Kaplan-Meier analysis was used to examine the prognostic value of LUCAT1 and CXCL2 in ccRCC patients. Loss and gain of function were performed to explore the effect of LUCAT1 on proliferation and invasion in ccRCC cells. Western blotting was performed to evaluate the underlying mechanisms of LUCAT1 in ccRCC progression. Chemokine stimulation assay was performed to investigate possible mechanisms controlling LUCAT1 expression in ccRCC cells. Enzyme-linked immunosorbent assays were performed to determine serum CXCL2 in ccRCC patients and healthy volunteers. Receiver operating characteristic curve analysis was performed to examine the clinical diagnostic value of serum CXCL2 in ccRCC. We found that LUCAT1 was significantly upregulated in both clin...Continue Reading

Citations

Feb 13, 2020·Journal of Cellular and Molecular Medicine·Cheng FangShiguo Zhu
Aug 20, 2020·Technology in Cancer Research & Treatment·Zhaoyu LiuYongheng Yang
May 10, 2019·Journal of Cellular Biochemistry·Seyed Ali MirhosseiniJafar Amani
Mar 27, 2019·Oxidative Medicine and Cellular Longevity·Xiaole WangJingcheng Dong
Dec 15, 2020·Cell Proliferation·Wenwen ZhangJinhai Tang
Dec 29, 2020·Biomedicine & Pharmacotherapy = Biomédecine & Pharmacothérapie·Ce XingFeng Bai

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