Long non-coding RNA LUCAT1 promotes tumourigenesis by inhibiting ANXA2 phosphorylation in hepatocellular carcinoma

Journal of Cellular and Molecular Medicine
Yun LouGuoqiang Li

Abstract

Long non-coding RNAs (lncRNAs) play essential roles in diverse biological processes; however, current understanding of the mechanism underlying the regulation of tumour proliferation and metastasis is limited. Lung cancer-associated transcript 1 (LUCAT1) has been reported in a variety of human cancers, while its role in hepatocellular carcinoma (HCC) remains unclear. This study aimed to determine the biological role and underlying mechanism of LUCAT1 on progression and metastasis in HCC cells and clinical specimens. Our results demonstrated that LUCAT1 was up-regulated in HCC tissues and cells. Loss- and gain-of-function studies revealed that LUCAT1 promotes the proliferation and metastasis of HCC cells in vitro and in vivo. Furthermore, RNA pulldown and Western blot assays indicated that LUCAT1 inhibited the phosphorylation of Annexin A2 (ANXA2) to reduce the degradation of ANXA2-S100A10 heterotetramer (AIIt), which in turn accelerated the secretion of plasminogen into plasmin, thereby resulting in the activation of metalloprotease proteins. In conclusion, we propose that LUCAT1 serves as a novel diagnostic and therapeutic target for HCC.

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Citations

Jun 2, 2020·World Journal of Gastroenterology : WJG·Li-Wei QiuHai-Jian Zhang
Aug 17, 2019·Cellular Signalling·Si-Nan SunTao Xu
Dec 29, 2020·Biomedicine & Pharmacotherapy = Biomédecine & Pharmacothérapie·Ce XingFeng Bai
Oct 24, 2019·Pathology, Research and Practice·Lining HuangXingming Jiang
Feb 6, 2021·Future Oncology·Min MaChangwei Lin
May 11, 2021·Frontiers in Oncology·Soudeh Ghafouri-FardMohammad Taheri
Aug 3, 2021·Frontiers in Cell and Developmental Biology·Lijun WangXuesong Jiang

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Methods Mentioned

BETA
transfection
flow cytometry
in vitro transcription
PCR
xenografts
xenograft
pulldown
immunoprecipitation
electrophoresis
ELISA

Software Mentioned

GraphPad
GraphPad Prism
Rapid
Cat

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