Long non-coding RNA MACC1-AS1 promoted pancreatic carcinoma progression through activation of PAX8/NOTCH1 signaling pathway

Journal of Experimental & Clinical Cancer Research : CR
Chen QiJiang Jianshuai

Abstract

Accumulated evidences have demonstrated that long non-coding RNAs (lncRNAs) are dysregulated and correlate with the pathophysiological basis of malignant tumors. The objective of this research is to uncover the possible molecular mechanism of MACC1-AS1 regarding the regulation of pancreatic carcinoma (PC) metastasis. lncRNA microarray and qRT-PCR were applied to identify differentially expressed lncRNA profile in PC. The function and role of MACC1-AS1 in PC were assessed via in vitro as well as in vivo assays. Luciferase analyses, RNA immunoprecipitation, and RNA pull-down were performed to determined the underlying MACC1-AS1 mechanisms. Numbers of differentially expressed lncRNAs in PC were identified via lncRNA microarrays, among which MACC1-AS1 was revealed as the most abundant lncRNA. The upregulation of MACC1-AS1 in PC was further confirmed in two expanded PC cohorts, which showed that MACC1-AS1 expression was upregulated in those PC patients with poor survival. Functionally, knockdown of MACC1-AS1 inhibited the proliferation as well as metastasis of PC cells. Meanwhile, MACC1-AS1 upregulated the expression of PAX8 protein, which promoted aerobic glycolysis and activated NOTCH1 signaling. Additionally, PAX8 was upregulated...Continue Reading

References

Jan 20, 2017·British Journal of Cancer·Siddhartha P KarKate Lawrenson
Apr 19, 2017·Pancreatology : Official Journal of the International Association of Pancreatology (IAP) ... [et Al.]·Lu-Lu ZhaiTong-Fa Ju
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Sep 25, 2017·Journal of Bone and Mineral Research : the Official Journal of the American Society for Bone and Mineral Research·Elizabeth S SilagiMakarand V Risbud
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Feb 5, 2019·Journal of Cellular Physiology·Lei Yue, Jing Guo

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Citations

Apr 28, 2020·The Journal of International Medical Research·Yuling GuoZhengyu Zhan
May 1, 2020·World Journal of Gastroenterology : WJG·Tao XuBin Niu
Mar 11, 2020·Frontiers in Oncology·Maria Lina TorneselloFranco Maria Buonaguro
Apr 11, 2020·Journal of Experimental & Clinical Cancer Research : CR·Zhonghou RongWenmao Geng

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Methods Mentioned

BETA
RNA-seq
pull-down
Assay
immunoprecipitation
RIP
Protein Assay
transfection
xenograft
electrophoresis
ubiquitination

Software Mentioned

Cytoscape
GTVision
GraphPad Prism
GeneSpring GX
ArrayStar Human LncRNA array
LifeScope

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