Long non-coding RNA Malat1 activated autophagy, hence promoting cell proliferation and inhibiting apoptosis by sponging miR-101 in colorectal cancer

Cellular & Molecular Biology Letters
Yaoran SiChunsheng Ding

Abstract

Long non-coding RNA Malat1 has been widely identified as an oncogene which shows a significant relationship with tumorigenesis in colorectal cancer (CRC). Nonetheless, whether Malat1 participates in the autophagy of colorectal cancer remains unclear. First, the expression level of Malat1 in 96 pairs of colorectal cancer tissues and four cell lines was detected by qRT-PCR. Subsequently, the autophagy activity in colorectal cancer tissues and cell lines was detected by western blot. Furthermore, the CCK-8 assay and flow cytometry (FCM) were performed to detect the role of autophagy activated by Malat1 in colorectal cancer cell lines. In this study, significantly increased Malat1 expression and autophagy activity were found in colorectal cancer tissues compared with the adjacent normal tissues. Also, the Malat1 level was positively correlated with the expression of LC3-II mRNA in vivo. Moreover, autophagy activation and cell proliferation were significantly facilitated by Malat1 in colorectal cancer cells, while apoptosis decreased. Above all, the inhibition of autophagy by 3-MA not only relieved the Malat1-induced cell proliferation but also promoted the Malat1-induced cell apoptosis. In addition, Malat1 was found to act as an en...Continue Reading

References

Mar 1, 1982·Proceedings of the National Academy of Sciences of the United States of America·P O Seglen, P B Gordon
Dec 2, 2000·Science·D J Klionsky, S D Emr
Sep 8, 2010·Cancer Letters·Dwayne G Stupack
Nov 19, 2011·Nature Reviews. Genetics·Manel Esteller
Nov 8, 2012·Nature Structural & Molecular Biology·Sònia Guil, Manel Esteller
Mar 27, 2013·Journal of Molecular Medicine : Official Organ of the Gesellschaft Deutscher Naturforscher Und Ärzte·Tony GutschnerSven Diederichs
Jun 1, 2013·BMC Biology·Aleksandra E KornienkoFlorian M Pauler
Jun 1, 2013·Journal of the American Society of Nephrology : JASN·Susanna Tomasoni, Ariela Benigni
Oct 10, 2013·Nature Reviews. Molecular Cell Biology·Sarah Geisler, Jeff Coller
Jan 23, 2014·Clinical Cancer Research : an Official Journal of the American Association for Cancer Research·Yu FanYong Liu
May 13, 2014·Asian Pacific Journal of Cancer Prevention : APJCP·Jiang-Hua LiuDian-Zhong Luo
Jul 8, 2014·Asian Pacific Journal of Cancer Prevention : APJCP·Jing SunYu-Xin Li
Oct 7, 2014·International Journal of Biological Sciences·Haijian WuYuan Hong
Dec 20, 2014·Cell Death and Differentiation·S ShaliniS Kumar
Mar 10, 2015·Molecular Cancer·Zhenyi SuQiang Yu
Apr 22, 2015·Oncogene·V W Rebecca, R K Amaravadi
Dec 17, 2015·Nature Reviews. Genetics·Jeffrey J Quinn, Howard Y Chang
May 4, 2016·Nature Reviews. Nephrology·Johan M Lorenzen, Thomas Thum
Aug 16, 2016·Biochemical and Biophysical Research Communications·Peng YuanJianghe Fan
Oct 6, 2017·Cell Death & Disease·Lixian YangHongchuan Jin

❮ Previous
Next ❯

Citations

Nov 14, 2019·International Journal of Molecular Sciences·Phillip J McCownJessica A Brown
Dec 24, 2019·Cellular & Molecular Biology Letters·Rafal Bartoszewski, Aleksander F Sikorski
Jul 15, 2020·Cancer Biology & Therapy·Anqi YanJichan Nie
Jan 12, 2021·Biochimica Et Biophysica Acta. Reviews on Cancer·Bela GoyalSubash Chandra Gupta
Jan 27, 2021·Digestive and Liver Disease : Official Journal of the Italian Society of Gastroenterology and the Italian Association for the Study of the Liver·Lan YuWei Luan
Feb 10, 2021·Cell Death Discovery·Na JiangLei Shang
Mar 25, 2021·Cell Biology International·Manjima Chatterjee, Pragasam Viswanathan
Apr 27, 2021·Pharmacology & Therapeutics·Li ChenHaibo Xu
Jun 19, 2021·Biochemical Pharmacology·Yaaqub Abiodun UthmanMurtala Bello Abubakar
Dec 1, 2020·Molecular Therapy Oncolytics·Hamed Mirzaei, Michael R Hamblin

❮ Previous
Next ❯

Methods Mentioned

BETA
PCR
transfection
electrophoresis
flow cytometry

Software Mentioned

Quantity One
SPSS

Related Concepts

Related Feeds

Apoptosis in Cancer

Apoptosis is an important mechanism in cancer. By evading apoptosis, tumors can continue to grow without regulation and metastasize systemically. Many therapies are evaluating the use of pro-apoptotic activation to eliminate cancer growth. Here is the latest research on apoptosis in cancer.

Autophagy & Model Organisms

Autophagy is a cellular process that allows degradation by the lysosome of cytoplasmic components such as proteins or organelles. Here is the latest research on autophagy & model organisms

Apoptosis

Apoptosis is a specific process that leads to programmed cell death through the activation of an evolutionary conserved intracellular pathway leading to pathognomic cellular changes distinct from cellular necrosis