Long non-coding RNA MEG3 suppresses survival, migration, and invasion of cervical cancer

OncoTargets and Therapy
Xiuhui Chen, Junying Qu

Abstract

Long non-coding RNAs nowadays emerge as important biomarkers or potential therapeutic targets discussed in human cancers. Among them, maternally expressed gene 3 (MEG3) is known to be decreased in a variety of malignancies, and this affects tumor cellular proliferation, migration, and invasion. Quantitative real-time PCR was performed to detect the expression of MEG3 in normal cervical epithelium, cervical intraepithelial neoplasia, and cervical squamous cell carcinoma tissues. Gain-of-function and loss-of-function studies were carried out to determine the effect of MEG3 on cell survival, migration, and invasion, which was evaluated by CCK-8 assay, wound healing assay, and transwell assays. mRNA and protein expression of Rac1 were finally determined by quantitative real-time PCR and immunoblotting, respectively. In addition, rescue experiments were performed by overexpression of Rac1. The expression of MEG3 was downregulated in cervical intraepithelial neoplasia and squamous cell carcinoma tissues. Forced expression of MEG3 led to reduced abilities of cell survival. Overexpression of MEG3 also inhibited cell migration and invasion in vitro. Cell proliferation marker and EMT markers were changed consistently with the phenotype. ...Continue Reading

Citations

May 8, 2020·Cancer Biotherapy & Radiopharmaceuticals·Xi PanPei-Guo Cao
Aug 16, 2019·World Journal of Gastroenterology : WJG·Wei WangQing-Chang Li
Dec 30, 2020·International Journal of Molecular Sciences·Miguel Ángel Cáceres-DuránAmanda Ferreira Vidal

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Methods Mentioned

BETA
GTPase
PCR

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